Potential modulation on BCRP and MRP 4 by onion: In vivo and ex-vivo studies

Yu Chin Lin; Chung Ping Yu; Shiuan Pey Lin; Pei Wen Hsu; Pei Dawn Lee Chao; Yu Chi Hou; Shin Hun Juang

doi:10.1016/j.jff.2014.03.023

Potential modulation on BCRP and MRP 4 by onion: In vivo and ex-vivo studies

Yu Chin Lin
, Chung Ping Yu
, Shiuan Pey Lin
, Pei Wen Hsu
, Pei Dawn Lee Chao
, Yu Chi Hou^*
, Shin Hun Juang

^*Corresponding author for this work

China Medical University Taichung

Research output: Contribution to journal › Journal Article › peer-review

3 Scopus citations

Abstract

Onion skin contains quercetin, kaempferol and their glycosides. Methotrexate (MTX), an immunosuppressant with narrow therapeutic window, is used as a probe substrate of BCRP and MRP 4. This study investigated the effect of onion skin powder (OSP) on MTX pharmacokinetics. Rats were orally administered MTX with and without OSP. The serum concentration of MTX was determined by fluorescence polarization immunoassay. Our results showed that after coadministration of 0.5 g/kg of OSP the AUC_0-3600 and MRT of MTX were significantly increased by 222% and 101%, whereas 1.0 g/kg of OSP significantly increased them by 126% and 82%, respectively. Cell studies showed that OSP metabolites inhibited the functions of BCRP and MRP 4, whereas OSP activated BCRP. Thus concomitant intake of onion skin increased the systemic exposure and toxicity of MTX through inhibition on BCRP and MRP 4.

Original language	English
Pages (from-to)	243-251
Number of pages	9
Journal	Journal of Functional Foods
Volume	8
Issue number	1
DOIs	https://doi.org/10.1016/j.jff.2014.03.023
State	Published - 2014
Externally published	Yes

Keywords

BCRP
MRP 4
Methotrexate
Onion

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10.1016/j.jff.2014.03.023

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@article{3ad4e0c5403043c2bcf5377c69d94219,

title = "Potential modulation on BCRP and MRP 4 by onion: In vivo and ex-vivo studies",

abstract = "Onion skin contains quercetin, kaempferol and their glycosides. Methotrexate (MTX), an immunosuppressant with narrow therapeutic window, is used as a probe substrate of BCRP and MRP 4. This study investigated the effect of onion skin powder (OSP) on MTX pharmacokinetics. Rats were orally administered MTX with and without OSP. The serum concentration of MTX was determined by fluorescence polarization immunoassay. Our results showed that after coadministration of 0.5 g/kg of OSP the AUC0-3600 and MRT of MTX were significantly increased by 222\% and 101\%, whereas 1.0 g/kg of OSP significantly increased them by 126\% and 82\%, respectively. Cell studies showed that OSP metabolites inhibited the functions of BCRP and MRP 4, whereas OSP activated BCRP. Thus concomitant intake of onion skin increased the systemic exposure and toxicity of MTX through inhibition on BCRP and MRP 4.",

keywords = "BCRP, MRP 4, Methotrexate, Onion",

author = "Lin, \{Yu Chin\} and Yu, \{Chung Ping\} and Lin, \{Shiuan Pey\} and Hsu, \{Pei Wen\} and Chao, \{Pei Dawn Lee\} and Hou, \{Yu Chi\} and Juang, \{Shin Hun\}",

year = "2014",

doi = "10.1016/j.jff.2014.03.023",

language = "英语",

volume = "8",

pages = "243--251",

journal = "Journal of Functional Foods",

issn = "1756-4646",

publisher = "Elsevier Ltd",

number = "1",

}

TY - JOUR

T1 - Potential modulation on BCRP and MRP 4 by onion

T2 - In vivo and ex-vivo studies

AU - Lin, Yu Chin

AU - Yu, Chung Ping

AU - Lin, Shiuan Pey

AU - Hsu, Pei Wen

AU - Chao, Pei Dawn Lee

AU - Hou, Yu Chi

AU - Juang, Shin Hun

PY - 2014

Y1 - 2014

N2 - Onion skin contains quercetin, kaempferol and their glycosides. Methotrexate (MTX), an immunosuppressant with narrow therapeutic window, is used as a probe substrate of BCRP and MRP 4. This study investigated the effect of onion skin powder (OSP) on MTX pharmacokinetics. Rats were orally administered MTX with and without OSP. The serum concentration of MTX was determined by fluorescence polarization immunoassay. Our results showed that after coadministration of 0.5 g/kg of OSP the AUC0-3600 and MRT of MTX were significantly increased by 222% and 101%, whereas 1.0 g/kg of OSP significantly increased them by 126% and 82%, respectively. Cell studies showed that OSP metabolites inhibited the functions of BCRP and MRP 4, whereas OSP activated BCRP. Thus concomitant intake of onion skin increased the systemic exposure and toxicity of MTX through inhibition on BCRP and MRP 4.

AB - Onion skin contains quercetin, kaempferol and their glycosides. Methotrexate (MTX), an immunosuppressant with narrow therapeutic window, is used as a probe substrate of BCRP and MRP 4. This study investigated the effect of onion skin powder (OSP) on MTX pharmacokinetics. Rats were orally administered MTX with and without OSP. The serum concentration of MTX was determined by fluorescence polarization immunoassay. Our results showed that after coadministration of 0.5 g/kg of OSP the AUC0-3600 and MRT of MTX were significantly increased by 222% and 101%, whereas 1.0 g/kg of OSP significantly increased them by 126% and 82%, respectively. Cell studies showed that OSP metabolites inhibited the functions of BCRP and MRP 4, whereas OSP activated BCRP. Thus concomitant intake of onion skin increased the systemic exposure and toxicity of MTX through inhibition on BCRP and MRP 4.

KW - BCRP

KW - MRP 4

KW - Methotrexate

KW - Onion

UR - https://www.scopus.com/pages/publications/84899908585

U2 - 10.1016/j.jff.2014.03.023

DO - 10.1016/j.jff.2014.03.023

M3 - 文章

AN - SCOPUS:84899908585

SN - 1756-4646

VL - 8

SP - 243

EP - 251

JO - Journal of Functional Foods

JF - Journal of Functional Foods

IS - 1

ER -

Potential modulation on BCRP and MRP 4 by onion: In vivo and ex-vivo studies

Abstract

Keywords

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