Potential modulation on P-glycoprotein and CYP3A by soymilk and miso: In vivo and ex-vivo studies

C. P. Yu; Y. W. Hsieh; S. P. Lin; Y. C. Chi; P. Hariharan; P. D.L. Chao; Y. C. Hou

doi:10.1016/j.foodchem.2013.10.058

Potential modulation on P-glycoprotein and CYP3A by soymilk and miso: In vivo and ex-vivo studies

C. P. Yu, Y. W. Hsieh, S. P. Lin, Y. C. Chi, P. Hariharan, P. D.L. Chao^*, Y. C. Hou

^*Corresponding author for this work

China Medical University Taichung

Research output: Contribution to journal › Journal Article › peer-review

13 Scopus citations

Abstract

P-glycoprotein (P-gp) and CYP3A4 both play very important roles in drug bioavailability, resistance and interactions. Our in vitro studies indicated that P-gp function was activated by many isoflavones. This study investigated the in vivo effects of soymilk and miso, isoflavone-rich soy foods, on P-gp and CYP3A by tracing the pharmacokinetics of cyclosporine (CSP), a probe drug of P-gp. Rats were orally administered CSP with and without soymilk or miso. A specific monoclonal fluorescence polarisation immunoassay was used to determine the blood concentration of CSP. The results showed that soymilk and miso significantly decreased the C_max of CSP by 64.5% and 78.3%, and reduced the AUC_0-540 by 64.9% and 78.3%, respectively. Mechanism studies revealed that the activities of P-gp and CYP3A4 were induced by soymilk and miso. In conclusion, ingestion of soymilk and miso significantly activated the functions of P-gp and CYP3A.

Original language	English
Pages (from-to)	25-30
Number of pages	6
Journal	Food Chemistry
Volume	149
DOIs	https://doi.org/10.1016/j.foodchem.2013.10.058
State	Published - 2014
Externally published	Yes

Keywords

CYP3A
Cyclosporine
Miso
P-gp
Soymilk powders

Access to Document

10.1016/j.foodchem.2013.10.058

Cite this

@article{22fe019bdf1743388bd5117c3aab91a6,

title = "Potential modulation on P-glycoprotein and CYP3A by soymilk and miso: In vivo and ex-vivo studies",

abstract = "P-glycoprotein (P-gp) and CYP3A4 both play very important roles in drug bioavailability, resistance and interactions. Our in vitro studies indicated that P-gp function was activated by many isoflavones. This study investigated the in vivo effects of soymilk and miso, isoflavone-rich soy foods, on P-gp and CYP3A by tracing the pharmacokinetics of cyclosporine (CSP), a probe drug of P-gp. Rats were orally administered CSP with and without soymilk or miso. A specific monoclonal fluorescence polarisation immunoassay was used to determine the blood concentration of CSP. The results showed that soymilk and miso significantly decreased the Cmax of CSP by 64.5% and 78.3%, and reduced the AUC0-540 by 64.9% and 78.3%, respectively. Mechanism studies revealed that the activities of P-gp and CYP3A4 were induced by soymilk and miso. In conclusion, ingestion of soymilk and miso significantly activated the functions of P-gp and CYP3A.",

keywords = "CYP3A, Cyclosporine, Miso, P-gp, Soymilk powders",

author = "Yu, {C. P.} and Hsieh, {Y. W.} and Lin, {S. P.} and Chi, {Y. C.} and P. Hariharan and Chao, {P. D.L.} and Hou, {Y. C.}",

year = "2014",

doi = "10.1016/j.foodchem.2013.10.058",

language = "英语",

volume = "149",

pages = "25--30",

journal = "Food Chemistry",

issn = "0308-8146",

publisher = "Elsevier Ltd",

}

TY - JOUR

T1 - Potential modulation on P-glycoprotein and CYP3A by soymilk and miso

T2 - In vivo and ex-vivo studies

AU - Yu, C. P.

AU - Hsieh, Y. W.

AU - Lin, S. P.

AU - Chi, Y. C.

AU - Hariharan, P.

AU - Chao, P. D.L.

AU - Hou, Y. C.

PY - 2014

Y1 - 2014

N2 - P-glycoprotein (P-gp) and CYP3A4 both play very important roles in drug bioavailability, resistance and interactions. Our in vitro studies indicated that P-gp function was activated by many isoflavones. This study investigated the in vivo effects of soymilk and miso, isoflavone-rich soy foods, on P-gp and CYP3A by tracing the pharmacokinetics of cyclosporine (CSP), a probe drug of P-gp. Rats were orally administered CSP with and without soymilk or miso. A specific monoclonal fluorescence polarisation immunoassay was used to determine the blood concentration of CSP. The results showed that soymilk and miso significantly decreased the Cmax of CSP by 64.5% and 78.3%, and reduced the AUC0-540 by 64.9% and 78.3%, respectively. Mechanism studies revealed that the activities of P-gp and CYP3A4 were induced by soymilk and miso. In conclusion, ingestion of soymilk and miso significantly activated the functions of P-gp and CYP3A.

AB - P-glycoprotein (P-gp) and CYP3A4 both play very important roles in drug bioavailability, resistance and interactions. Our in vitro studies indicated that P-gp function was activated by many isoflavones. This study investigated the in vivo effects of soymilk and miso, isoflavone-rich soy foods, on P-gp and CYP3A by tracing the pharmacokinetics of cyclosporine (CSP), a probe drug of P-gp. Rats were orally administered CSP with and without soymilk or miso. A specific monoclonal fluorescence polarisation immunoassay was used to determine the blood concentration of CSP. The results showed that soymilk and miso significantly decreased the Cmax of CSP by 64.5% and 78.3%, and reduced the AUC0-540 by 64.9% and 78.3%, respectively. Mechanism studies revealed that the activities of P-gp and CYP3A4 were induced by soymilk and miso. In conclusion, ingestion of soymilk and miso significantly activated the functions of P-gp and CYP3A.

KW - CYP3A

KW - Cyclosporine

KW - Miso

KW - P-gp

KW - Soymilk powders

UR - http://www.scopus.com/inward/record.url?scp=84887263949&partnerID=8YFLogxK

U2 - 10.1016/j.foodchem.2013.10.058

DO - 10.1016/j.foodchem.2013.10.058

M3 - 文章

C2 - 24295672

AN - SCOPUS:84887263949

SN - 0308-8146

VL - 149

SP - 25

EP - 30

JO - Food Chemistry

JF - Food Chemistry

ER -

Potential modulation on P-glycoprotein and CYP3A by soymilk and miso: In vivo and ex-vivo studies

Abstract

Keywords

Access to Document

Other files and links

Fingerprint

Cite this