Potential negative regulation of damage-recognition proteins in cisplatin-resistant HeLa cells in response to DNA damage.

Research output: Contribution to journalJournal Article peer-review

Abstract

Nuclear proteins in cisplatin-resistant (CPR) and non-resistant HeLa cells were assayed by 2-dimensional polyacrylamide gel electrophoresis. The levels of at least 4 suppressible nuclear proteins were reduced in CPR cells. These proteins were partially restored in CPR revertants that are known to be less capable of repairing cisplatin-DNA adducts. Southwestern blotting assays indicate that in CPR cells at least 1 DNA-binding protein was suppressed, and concomitantly with it several damage-recognition proteins (DRPs) were induced. In addition, gel mobility shift analysis shows that DRPs were overexpressed in CPR cells. These results suggest a potential negative regulation in normal cells that may be important for the induction of DRPs and the emergence of a repair-mediated CPR phenotype.
Original languageAmerican English
Pages (from-to)59-66
JournalMutation research
Volume264
Issue number2
DOIs
StatePublished - 1991

Keywords

  • Cisplatin/pharmacology
  • DNA Damage
  • DNA Repair
  • DNA-Binding Proteins/metabolism
  • Drug Resistance
  • Electrophoresis, Gel, Two-Dimensional
  • Gene Expression
  • HeLa Cells
  • Humans
  • Nuclear Proteins/metabolism

Fingerprint

Dive into the research topics of 'Potential negative regulation of damage-recognition proteins in cisplatin-resistant HeLa cells in response to DNA damage.'. Together they form a unique fingerprint.

Cite this