Abstract
Nuclear proteins in cisplatin-resistant (CPR) and non-resistant HeLa cells were assayed by 2-dimensional polyacrylamide gel electrophoresis. The levels of at least 4 suppressible nuclear proteins were reduced in CPR cells. These proteins were partially restored in CPR revertants that are known to be less capable of repairing cisplatin-DNA adducts. Southwestern blotting assays indicate that in CPR cells at least 1 DNA-binding protein was suppressed, and concomitantly with it several damage-recognition proteins (DRPs) were induced. In addition, gel mobility shift analysis shows that DRPs were overexpressed in CPR cells. These results suggest a potential negative regulation in normal cells that may be important for the induction of DRPs and the emergence of a repair-mediated CPR phenotype.
Original language | American English |
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Pages (from-to) | 59-66 |
Journal | Mutation research |
Volume | 264 |
Issue number | 2 |
DOIs | |
State | Published - 1991 |
Keywords
- Cisplatin/pharmacology
- DNA Damage
- DNA Repair
- DNA-Binding Proteins/metabolism
- Drug Resistance
- Electrophoresis, Gel, Two-Dimensional
- Gene Expression
- HeLa Cells
- Humans
- Nuclear Proteins/metabolism