Prenatal protein malnutrition effects on the serotonergic system in the hippocampal formation: An immunocytochemical, ligand binding, and neurochemical study

Gene J. Blatt; Chen Jin-Chung; Douglas L. Rosene; Ladislav Volicer; Janina R. Galler

doi:10.1016/0361-9230(94)90025-6

Prenatal protein malnutrition effects on the serotonergic system in the hippocampal formation: An immunocytochemical, ligand binding, and neurochemical study

Gene J. Blatt^*, Chen Jin-Chung, Douglas L. Rosene, Ladislav Volicer, Janina R. Galler

^*Corresponding author for this work

Research output: Contribution to journal › Journal Article › peer-review

54 Scopus citations

Abstract

Prenatally protein malnourished rats bom to dams maintained on a 6% casein diet during pregnancy and then fostered at birth to females on a 25% casein diet show adult alterations in hippocampal kindling and longterm potentiation and behavioral changes that all suggest dysfunction of hippocampal formation (HF). In the present investigation, compared to well-nourished controls, 220 day malnourished rats exhibited a decrease in the 5-HT fiber density in the dentate gyrus (DG) and CA3 subfield and, a 15-25% decrease 5-HT uptake sites assayed with [³H]-citalopram in CA3 andCA1. In malnourished rats, 5-HT_1A receptors assayed with [³H]8-OH-DPAT were decreased by 20% in CA3. Because most hippocampal subfields showed no 5-HT changes, hippocampal 5-HT levels determined via HPLC methods were similar in adult malnourished and control rats. These results suggest that there are localized changes in the 5-HT afferent system in the hippocampal formation of the 220 day prenatally protein malnourished rat. Considering the 5-HT afferent input to inhibitory intrahippocampal neurons, the decreased 5-HT plexus may result in increased inhibition within specific hippocampal subfields despite overall normal levels of 5-HT in the total HF.

Original language	English
Pages (from-to)	507-518
Number of pages	12
Journal	Brain Research Bulletin
Volume	34
Issue number	5
DOIs	https://doi.org/10.1016/0361-9230(94)90025-6
State	Published - 1994
Externally published	Yes

Keywords

(5-HT)
HPLC
Hippocampal formation
Immunocytochemistry
On-the-slide in vitro ligand binding
Prenatal protein malnutrition
Serotonin

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/0361-9230(94)90025-6

Cite this

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title = "Prenatal protein malnutrition effects on the serotonergic system in the hippocampal formation: An immunocytochemical, ligand binding, and neurochemical study",

abstract = "Prenatally protein malnourished rats bom to dams maintained on a 6% casein diet during pregnancy and then fostered at birth to females on a 25% casein diet show adult alterations in hippocampal kindling and longterm potentiation and behavioral changes that all suggest dysfunction of hippocampal formation (HF). In the present investigation, compared to well-nourished controls, 220 day malnourished rats exhibited a decrease in the 5-HT fiber density in the dentate gyrus (DG) and CA3 subfield and, a 15-25% decrease 5-HT uptake sites assayed with [3H]-citalopram in CA3 andCA1. In malnourished rats, 5-HT1A receptors assayed with [3H]8-OH-DPAT were decreased by 20% in CA3. Because most hippocampal subfields showed no 5-HT changes, hippocampal 5-HT levels determined via HPLC methods were similar in adult malnourished and control rats. These results suggest that there are localized changes in the 5-HT afferent system in the hippocampal formation of the 220 day prenatally protein malnourished rat. Considering the 5-HT afferent input to inhibitory intrahippocampal neurons, the decreased 5-HT plexus may result in increased inhibition within specific hippocampal subfields despite overall normal levels of 5-HT in the total HF.",

keywords = "(5-HT), HPLC, Hippocampal formation, Immunocytochemistry, On-the-slide in vitro ligand binding, Prenatal protein malnutrition, Serotonin",

author = "Blatt, {Gene J.} and Chen Jin-Chung and Rosene, {Douglas L.} and Ladislav Volicer and Galler, {Janina R.}",

year = "1994",

doi = "10.1016/0361-9230(94)90025-6",

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pages = "507--518",

journal = "Brain Research Bulletin",

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T1 - Prenatal protein malnutrition effects on the serotonergic system in the hippocampal formation

T2 - An immunocytochemical, ligand binding, and neurochemical study

AU - Blatt, Gene J.

AU - Jin-Chung, Chen

AU - Rosene, Douglas L.

AU - Volicer, Ladislav

AU - Galler, Janina R.

PY - 1994

Y1 - 1994

N2 - Prenatally protein malnourished rats bom to dams maintained on a 6% casein diet during pregnancy and then fostered at birth to females on a 25% casein diet show adult alterations in hippocampal kindling and longterm potentiation and behavioral changes that all suggest dysfunction of hippocampal formation (HF). In the present investigation, compared to well-nourished controls, 220 day malnourished rats exhibited a decrease in the 5-HT fiber density in the dentate gyrus (DG) and CA3 subfield and, a 15-25% decrease 5-HT uptake sites assayed with [3H]-citalopram in CA3 andCA1. In malnourished rats, 5-HT1A receptors assayed with [3H]8-OH-DPAT were decreased by 20% in CA3. Because most hippocampal subfields showed no 5-HT changes, hippocampal 5-HT levels determined via HPLC methods were similar in adult malnourished and control rats. These results suggest that there are localized changes in the 5-HT afferent system in the hippocampal formation of the 220 day prenatally protein malnourished rat. Considering the 5-HT afferent input to inhibitory intrahippocampal neurons, the decreased 5-HT plexus may result in increased inhibition within specific hippocampal subfields despite overall normal levels of 5-HT in the total HF.

AB - Prenatally protein malnourished rats bom to dams maintained on a 6% casein diet during pregnancy and then fostered at birth to females on a 25% casein diet show adult alterations in hippocampal kindling and longterm potentiation and behavioral changes that all suggest dysfunction of hippocampal formation (HF). In the present investigation, compared to well-nourished controls, 220 day malnourished rats exhibited a decrease in the 5-HT fiber density in the dentate gyrus (DG) and CA3 subfield and, a 15-25% decrease 5-HT uptake sites assayed with [3H]-citalopram in CA3 andCA1. In malnourished rats, 5-HT1A receptors assayed with [3H]8-OH-DPAT were decreased by 20% in CA3. Because most hippocampal subfields showed no 5-HT changes, hippocampal 5-HT levels determined via HPLC methods were similar in adult malnourished and control rats. These results suggest that there are localized changes in the 5-HT afferent system in the hippocampal formation of the 220 day prenatally protein malnourished rat. Considering the 5-HT afferent input to inhibitory intrahippocampal neurons, the decreased 5-HT plexus may result in increased inhibition within specific hippocampal subfields despite overall normal levels of 5-HT in the total HF.

KW - (5-HT)

KW - HPLC

KW - Hippocampal formation

KW - Immunocytochemistry

KW - On-the-slide in vitro ligand binding

KW - Prenatal protein malnutrition

KW - Serotonin

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IS - 5

ER -

Prenatal protein malnutrition effects on the serotonergic system in the hippocampal formation: An immunocytochemical, ligand binding, and neurochemical study

Abstract

Keywords

UN SDGs

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