Preparation of biomimetic nanofibers by electrospinning of blends of silk fibroin and chitosan for bone tissue engineering

Shih Hsien Chen*, Guo Jyun Lai, Jyh Ping Chen

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

1 Scopus citations

Abstract

In this study, we have successfully fabricated electrospun bead-free silk fibroin (SF)/chitosan (CS) blend nanofibers (NFs) covering the whole range of CS content (0, 25, 50, 75, and 100%). NFs are treated with ethanol/ammonia aqueous solution to remove residual TFA and to change SF protein conformation. After the chemical treatment, SF/CS NFs could maintain the original structure for up to 54 days in culture medium. Human fetal osteoblast progenitor (hFOB) cells were capable of proliferating and osteogenic differentiating on SF/CS NFs from MTS and alkaline phosphatase assays. SF enhanced hFOB proliferation while CS enhanced hFOB differentiation. The prepared SF/CS blend NFs will be suitable for tissue engineering applications.

Original languageEnglish
Title of host publication4th IEEE International NanoElectronics Conference, INEC 2011
DOIs
StatePublished - 2011
Event4th IEEE International Nanoelectronics Conference, INEC 2011 - Tao-Yuan, Taiwan
Duration: 21 06 201124 06 2011

Publication series

NameProceedings - International NanoElectronics Conference, INEC
ISSN (Print)2159-3523

Conference

Conference4th IEEE International Nanoelectronics Conference, INEC 2011
Country/TerritoryTaiwan
CityTao-Yuan
Period21/06/1124/06/11

Keywords

  • chitosan
  • electrospinning
  • nanofibers
  • silk fibroin
  • tissue engineering

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