Preparation of PLLA membranes with different morphologies for culture of MG-63 Cells

Hwa Chang Liu, I. Chi Lee, Jyh Horng Wang, Shu Hua Yang, Tai Horng Young*

*Corresponding author for this work

Research output: Contribution to journalJournal Article peer-review

74 Scopus citations


In this work, poly L-lactide (PLLA) membranes with different morphologies were prepared and the equilibrium phase diagram of membrane formation system of ethanol, methylene chloride, and PLLA was studied. Based on the phase diagram, particulate and porous membranes, dominated by crystallization and liquid-liquid demixing, respectively, could be prepared by changing the PLLA concentration of casting solution. In addition, in vitro interaction of MG-63 osteosarcoma cells and PLLA membranes with dense, porous and particulate morphologies was investigated. It was found that the particulate membrane not only could improve cell adhesion and growth, but also could upregulate the osteoblastic phenotype. Therefore, the PLLA membrane with particulate morphology satisfies the biomaterial requirement necessary for temporary scaffold to transplanted osteoblasts and provides a means for the architectural design of more complex tissue-engineered systems.

Original languageEnglish
Pages (from-to)4047-4056
Number of pages10
Issue number18
StatePublished - 08 2004
Externally publishedYes


  • MG-63 cells
  • PLLA
  • Particulate membranes


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