Pretreatment of lipase PS on enantioselective transesterification of (R,S)-ethyl 2-Hydroxyl-4-phenylbutyrate in lsooctane

Song Hui Huang, Shau Wei Tsai*

*Corresponding author for this work

Research output: Contribution to journalJournal Article peer-review

1 Scopus citations

Abstract

In the enantioselective transesterification of (R,S)-ethyl 2-hydroxy-4-phenylbutyrate with vinyl acetate in isooctane, Lipase PS pretreated with calcium hydride in iso-octane for 9 h at 4°C and that lyophilized for 8 h were found to be effective in decreasing the hydrolysis side-reaction and enhancing the productivity and optical purity of the remaining (R)-substrate. Anhydrous magnesium sulfate and calcium chloride were not found to be suitable as additives for controlling the water content of the lipase, owing to the low depletion rate of water. Moreover, the very low lipase activity was attributed to aggregation between Lipase PS and calcium chloride.

Original languageEnglish
Pages (from-to)161-166
Number of pages6
JournalJournal of the Chinese Institute of Chemical Engineers
Volume35
Issue number2
StatePublished - 03 2004
Externally publishedYes

Keywords

  • (R)-ethyl 2-hydroxy 4-phenylbutyrate
  • Hydrolysis
  • Kinetic resolution
  • Lipase
  • Transesterification

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