Probiotics have clinical, microbiologic, and immunologic efficacy in acute infectious diarrhea

Chien Chang Chen, Man Shan Kong, Ming Wei Lai, Hsun Chin Chao, Kuei Wen Chang, Shih Yen Chen, Yhu Chering Huang, Cheng Hsun Chiu, Wen Chen Li, Pen Yi Lin, Chih Jung Chen, Tzou Yien Lin*

*Corresponding author for this work

Research output: Contribution to journalJournal Article peer-review

92 Scopus citations


Background: Acute infectious diarrhea is a major cause of childhood morbidity and economic burden for families. We evaluate the clinical, microbiologic, and immunologic effects of probiotics in acute infectious diarrhea. Methods: Children (n = 304) aged 3 months to 6 years hospitalized for acute diarrhea were randomized to receive Bio-three (a mixture of Bacillus mesentericus, Enterococcus faecalis, and Clostridium butyricum) or placebo orally 3 times daily for 7 days. Fecal samples were homogenized for bacterial culture and blood cells were isolated for cell culture and cytokine analysis. This study is registered (NCT00463190). Results: The mean duration of diarrhea after start of therapy was 60.1 hours in the probiotics group versus 86.3 hours in the placebo group (P = 0.003). Hospital stay was shorter in the probiotics group than in the placebo group (P = 0.009). Counts of Bifidobacteria and Lactobacillus species were elevated in stool culture of the probiotics (Bio-three) group. IL-10 was increased in the serum and supernatants of cell culture in the probiotics group, and tumor necrosis factor-α values were down-regulated. Interferon-γ and IL-12 were mildly elevated in the probiotics group, compared with the placebo group. Conclusions: This probiotics mixture reduced the severity of diarrhea and length of hospital stay in children with acute diarrhea. In addition to restoring beneficial intestinal flora, probiotics may enhance host protective immunity such as down-regulation of pro-inflammatory cytokines and up-regulation of anti-inflammatory cytokines.

Original languageEnglish
Pages (from-to)135-138
Number of pages4
JournalPediatric Infectious Disease Journal
Issue number2
StatePublished - 02 2010


  • Bacillus mesentericus
  • Bio-three
  • Clostridium butyricum
  • Enterococcus faecalis
  • Peripheral blood mononuclear cells
  • Probiotics


Dive into the research topics of 'Probiotics have clinical, microbiologic, and immunologic efficacy in acute infectious diarrhea'. Together they form a unique fingerprint.

Cite this