Proteomic analysis of rat plasma following transient focal cerebral ischemia

Ruoli Chen*, Iolanda Vendrell, Carl Pc Chen, Diana Cash, Kim Galley O'Toole, Steve A. Williams, Christopher Jones, Jane E. Preston, Jun X. Wheeler

*Corresponding author for this work

Research output: Contribution to journalJournal Article peer-review

16 Scopus citations

Abstract

Aim: This study aimed to identify plasma protein changes in a rat model of ischemic stroke using a proteomic approach. Materials & Methods: Four male Sprague-Dawley rats (3-6 months old) were subjected to 90 min of left middle cerebral artery occlusion under anesthesia with 1.5% isoflurane in O 2/air followed by 24-h reperfusion. Blood samples (∼100 l) were collected at baseline, at the end of 90-min middle cerebral artery occlusion and at 24-h postreperfusion. Brain injuries were assessed by MRI at 24-h postreperfusion. Quantitative comparison of global plasma protein expression was performed using 2D differential in-gel electrophoresis. Differentially expressed protein spots were identified using peptide sequencing tandem mass spectrometry. Results: These rats had clear brain infarction in the left hemisphere detected by MRI. Thirty-three protein spots of plasma samples were differentially expressed following focal cerebral ischemia/reperfusion. These protein spots belonged to eight proteins. Six of them (α2-macroglobulin, complement C3, inter-α- trypsin inhibitor heavy chain H3, serum albumin, haptoglobin and transthyretin), which are a class of acute-phase proteins, changed significantly. Conclusion: This study describes the responses of young rats to focal cerebral ischemia and suggests that future studies should use aged animals to better mimic the clinical ischemic stroke setting.

Original languageEnglish
Pages (from-to)837-846
Number of pages10
JournalBiomarkers in Medicine
Volume5
Issue number6
DOIs
StatePublished - 12 2011

Keywords

  • acute-phase proteins
  • cerebral ischemia
  • plasma
  • proteomics
  • reperfusion

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