Purification, crystallization and preliminary X-ray crystallographic analysis of the phosphatase domain (PA3346PD) of the response regulator PA3346 from Pseudomonas aeruginosa PAO1

Li Ying Chen; Pei Hsun Wu; Hong Hsiang Guan; Hoong Kun Fun; Hwan You Chang; Chun Jung Chen

doi:10.1107/S2053230X15004197

Purification, crystallization and preliminary X-ray crystallographic analysis of the phosphatase domain (PA3346PD) of the response regulator PA3346 from Pseudomonas aeruginosa PAO1

Li Ying Chen, Pei Hsun Wu, Hong Hsiang Guan, Hoong Kun Fun, Hwan You Chang, Chun Jung Chen^*

^*Corresponding author for this work

Research output: Contribution to journal › Journal Article › peer-review

Abstract

The phosphatase domain (PA3346PD) of the response regulator PA3346 modulates the downstream anti-anti-σ factor PA3347 to regulate swarming motility in Pseudomonas aeruginosa PAO1. PA3346PD, which comprises the protein phosphatase 2C domain (PP2C), is classified as a Ser/Thr phosphatase of the Mg²⁺-or Mn²⁺-dependent protein phosphatase (PPM) family. The recombinant PA3346PD, with molecular mass 26 kDa, was overexpressed in Escherichia coli, purified on an Ni²⁺-NTA agarose column and crystallized by the sitting-drop vapour-diffusion method. X-ray diffraction data were collected from PA3346PD crystals to a resolution of 2.58 Å and the crystals belonged to space group I4132 or I4332, with unit-cell parameter a = 157.61 Å. Preliminary analysis indicates the presence of a monomer of PA3346PD in the asymmetric unit with a solvent content of 58.4%.

Original language	English
Pages (from-to)	434-437
Number of pages	4
Journal	Acta Crystallographica Section F: Structural Biology Communications
Volume	71
DOIs	https://doi.org/10.1107/S2053230X15004197
State	Published - 01 04 2015
Externally published	Yes

Bibliographical note

Publisher Copyright:
© 2015 International Union of Crystallography.

Keywords

Pseudomonas aeruginosa
phosphatase domain
response regulator

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10.1107/S2053230X15004197

Cite this

Chen, L. Y., Wu, P. H., Guan, H. H., Fun, H. K., Chang, H. Y., & Chen, C. J. (2015). Purification, crystallization and preliminary X-ray crystallographic analysis of the phosphatase domain (PA3346PD) of the response regulator PA3346 from Pseudomonas aeruginosa PAO1. Acta Crystallographica Section F: Structural Biology Communications, 71, 434-437. https://doi.org/10.1107/S2053230X15004197

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title = "Purification, crystallization and preliminary X-ray crystallographic analysis of the phosphatase domain (PA3346PD) of the response regulator PA3346 from Pseudomonas aeruginosa PAO1",

abstract = "The phosphatase domain (PA3346PD) of the response regulator PA3346 modulates the downstream anti-anti-σ factor PA3347 to regulate swarming motility in Pseudomonas aeruginosa PAO1. PA3346PD, which comprises the protein phosphatase 2C domain (PP2C), is classified as a Ser/Thr phosphatase of the Mg2+-or Mn2+-dependent protein phosphatase (PPM) family. The recombinant PA3346PD, with molecular mass 26 kDa, was overexpressed in Escherichia coli, purified on an Ni2+-NTA agarose column and crystallized by the sitting-drop vapour-diffusion method. X-ray diffraction data were collected from PA3346PD crystals to a resolution of 2.58 {\AA} and the crystals belonged to space group I4132 or I4332, with unit-cell parameter a = 157.61 {\AA}. Preliminary analysis indicates the presence of a monomer of PA3346PD in the asymmetric unit with a solvent content of 58.4%.",

keywords = "Pseudomonas aeruginosa, phosphatase domain, response regulator",

author = "Chen, {Li Ying} and Wu, {Pei Hsun} and Guan, {Hong Hsiang} and Fun, {Hoong Kun} and Chang, {Hwan You} and Chen, {Chun Jung}",

note = "Publisher Copyright: {\textcopyright} 2015 International Union of Crystallography.",

year = "2015",

month = apr,

day = "1",

doi = "10.1107/S2053230X15004197",

language = "英语",

volume = "71",

pages = "434--437",

journal = "Acta Crystallographica Section F: Structural Biology Communications",

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Chen, LY, Wu, PH, Guan, HH, Fun, HK, Chang, HY & Chen, CJ 2015, 'Purification, crystallization and preliminary X-ray crystallographic analysis of the phosphatase domain (PA3346PD) of the response regulator PA3346 from Pseudomonas aeruginosa PAO1', Acta Crystallographica Section F: Structural Biology Communications, vol. 71, pp. 434-437. https://doi.org/10.1107/S2053230X15004197

Purification, crystallization and preliminary X-ray crystallographic analysis of the phosphatase domain (PA3346PD) of the response regulator PA3346 from Pseudomonas aeruginosa PAO1. / Chen, Li Ying; Wu, Pei Hsun; Guan, Hong Hsiang et al.
In: Acta Crystallographica Section F: Structural Biology Communications, Vol. 71, 01.04.2015, p. 434-437.

Research output: Contribution to journal › Journal Article › peer-review

TY - JOUR

T1 - Purification, crystallization and preliminary X-ray crystallographic analysis of the phosphatase domain (PA3346PD) of the response regulator PA3346 from Pseudomonas aeruginosa PAO1

AU - Chen, Li Ying

AU - Wu, Pei Hsun

AU - Guan, Hong Hsiang

AU - Fun, Hoong Kun

AU - Chang, Hwan You

AU - Chen, Chun Jung

PY - 2015/4/1

Y1 - 2015/4/1

N2 - The phosphatase domain (PA3346PD) of the response regulator PA3346 modulates the downstream anti-anti-σ factor PA3347 to regulate swarming motility in Pseudomonas aeruginosa PAO1. PA3346PD, which comprises the protein phosphatase 2C domain (PP2C), is classified as a Ser/Thr phosphatase of the Mg2+-or Mn2+-dependent protein phosphatase (PPM) family. The recombinant PA3346PD, with molecular mass 26 kDa, was overexpressed in Escherichia coli, purified on an Ni2+-NTA agarose column and crystallized by the sitting-drop vapour-diffusion method. X-ray diffraction data were collected from PA3346PD crystals to a resolution of 2.58 Å and the crystals belonged to space group I4132 or I4332, with unit-cell parameter a = 157.61 Å. Preliminary analysis indicates the presence of a monomer of PA3346PD in the asymmetric unit with a solvent content of 58.4%.

AB - The phosphatase domain (PA3346PD) of the response regulator PA3346 modulates the downstream anti-anti-σ factor PA3347 to regulate swarming motility in Pseudomonas aeruginosa PAO1. PA3346PD, which comprises the protein phosphatase 2C domain (PP2C), is classified as a Ser/Thr phosphatase of the Mg2+-or Mn2+-dependent protein phosphatase (PPM) family. The recombinant PA3346PD, with molecular mass 26 kDa, was overexpressed in Escherichia coli, purified on an Ni2+-NTA agarose column and crystallized by the sitting-drop vapour-diffusion method. X-ray diffraction data were collected from PA3346PD crystals to a resolution of 2.58 Å and the crystals belonged to space group I4132 or I4332, with unit-cell parameter a = 157.61 Å. Preliminary analysis indicates the presence of a monomer of PA3346PD in the asymmetric unit with a solvent content of 58.4%.

KW - Pseudomonas aeruginosa

KW - phosphatase domain

KW - response regulator

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SN - 2053-230X

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JO - Acta Crystallographica Section F: Structural Biology Communications

JF - Acta Crystallographica Section F: Structural Biology Communications

ER -

Purification, crystallization and preliminary X-ray crystallographic analysis of the phosphatase domain (PA3346PD) of the response regulator PA3346 from Pseudomonas aeruginosa PAO1

Abstract

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Keywords

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