Purification of surfactin in pretreated fermentation broths by adsorptive removal of impurities

Huei Li Chen, Yi Shu Lee, Yu Hong Wei, Ruey Shin Juang*

*Corresponding author for this work

Research output: Contribution to journalJournal Article peer-review

39 Scopus citations


An attempt was made to further improve the purity of surfactin in the fermentation broth of Bacillus subtilis ATCC 21332 culture by adsorption or ion exchange after the broth had been treated by a two-stage ultrafiltration process. The commonly used neutral adsorption resin (macroporous XAD-7) and charged ion-exchange resin (AG1-X4) were adopted. Under the conditions tested the impurities in the treated broth such as macromolecules (proteins, polysaccharides, peptides) or smaller molecules (glycine, serine, threonine, alanine) were adsorbed or exchanged more quickly and preferably within 5 h of operation, purification of surfactin was hence achieved by adsorption removal of the impurities. It was shown that XAD-7 resins gave higher purity of surfactin than AG1-X4 resins. The recovery of surfactin from the remaining solution after adsorption using XAD-7 resin exceeded 95% at pH 6.5 and the purity increased from 76% (the feed) to 88%; however, the purity of surfactin reached up to 80% only after ion exchange using AG1-X4 resin. Finally, the breakthrough phenomena of surfactin solutions in a XAD-7 column were tested and discussed.

Original languageEnglish
Pages (from-to)452-459
Number of pages8
JournalBiochemical Engineering Journal
Issue number3
StatePublished - 01 07 2008
Externally publishedYes


  • Adsorption
  • Ion exchange
  • Pretreated fermentation broths
  • Surfactin purification
  • Ultrafiltration


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