Quality of placental RNA: Effects of explant size and culture duration

O. Brew; E. Nikolopoulou; A. Hughes; M. Christian; Y. Lee; O. Oduwole; M. H.F. Sullivan; A. Woodman

doi:10.1016/j.placenta.2016.08.083

Quality of placental RNA: Effects of explant size and culture duration

O. Brew^*
, E. Nikolopoulou
, A. Hughes
, M. Christian
, Y. Lee
, O. Oduwole
, M. H.F. Sullivan
, A. Woodman

^*Corresponding author for this work

Research output: Contribution to journal › Journal Article › peer-review

7 Scopus citations

Abstract

We evaluated the impact of placental micro (≤50 mg) and macro (∼200 mg) explants, oxygen concentration and culture method on placental RNA quality after long-term culture. Our findings show that micro explants cultured at 8% oxygen have the best RNA quality and tissue structure. Macro explants were less viable after long-term culture. Macro explants and explants undergoing syncytial degeneration produced poor quality RNA and should be avoided.

Original language	English
Pages (from-to)	45-48
Number of pages	4
Journal	Placenta
Volume	46
DOIs	https://doi.org/10.1016/j.placenta.2016.08.083
State	Published - 01 10 2016
Externally published	Yes

Bibliographical note

Publisher Copyright:
© 2016 Elsevier Ltd

Keywords

Explant viability
Macro explants
Micro explant
Oxygen concentration
Placenta
RNA quality
Syncytial regeneration

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10.1016/j.placenta.2016.08.083

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title = "Quality of placental RNA: Effects of explant size and culture duration",

abstract = "We evaluated the impact of placental micro (≤50 mg) and macro (∼200 mg) explants, oxygen concentration and culture method on placental RNA quality after long-term culture. Our findings show that micro explants cultured at 8\% oxygen have the best RNA quality and tissue structure. Macro explants were less viable after long-term culture. Macro explants and explants undergoing syncytial degeneration produced poor quality RNA and should be avoided.",

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doi = "10.1016/j.placenta.2016.08.083",

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T2 - Effects of explant size and culture duration

AU - Brew, O.

AU - Nikolopoulou, E.

AU - Hughes, A.

AU - Christian, M.

AU - Lee, Y.

AU - Oduwole, O.

AU - Sullivan, M. H.F.

AU - Woodman, A.

PY - 2016/10/1

Y1 - 2016/10/1

N2 - We evaluated the impact of placental micro (≤50 mg) and macro (∼200 mg) explants, oxygen concentration and culture method on placental RNA quality after long-term culture. Our findings show that micro explants cultured at 8% oxygen have the best RNA quality and tissue structure. Macro explants were less viable after long-term culture. Macro explants and explants undergoing syncytial degeneration produced poor quality RNA and should be avoided.

AB - We evaluated the impact of placental micro (≤50 mg) and macro (∼200 mg) explants, oxygen concentration and culture method on placental RNA quality after long-term culture. Our findings show that micro explants cultured at 8% oxygen have the best RNA quality and tissue structure. Macro explants were less viable after long-term culture. Macro explants and explants undergoing syncytial degeneration produced poor quality RNA and should be avoided.

KW - Explant viability

KW - Macro explants

KW - Micro explant

KW - Oxygen concentration

KW - Placenta

KW - RNA quality

KW - Syncytial regeneration

UR - https://www.scopus.com/pages/publications/84985017265

U2 - 10.1016/j.placenta.2016.08.083

DO - 10.1016/j.placenta.2016.08.083

M3 - 文章

C2 - 27697221

AN - SCOPUS:84985017265

SN - 0143-4004

VL - 46

SP - 45

EP - 48

JO - Placenta

JF - Placenta

ER -

Quality of placental RNA: Effects of explant size and culture duration

Abstract

Bibliographical note

Keywords

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