Quantification of urinary 8-iso-prostaglandin F using liquid chromatography-tandem mass spectrometry during cardiac valve surgery

Yun Hui Teng, Chih Wei Wang, Yun Tsan Liao, Min Wen Yang, Tsung Yun Liu*

*Corresponding author for this work

Research output: Contribution to journalJournal Article peer-review

7 Scopus citations

Abstract

Oxidative stress is an unavoidable event during many complex surgical procedures. 8-iso-prostaglandin F (8-iso-PGF ) is a reliable biomarker for the evaluation of oxidative stress in vivo. The aim of this study is to develop simple and accurate liquid chromatography-tandem mass spectrometry (LC-MS/MS) methods for the detection of urinary 8-iso-PGF in samples collected from patients who received a cardiopulmonary bypass (CPB) during cardiac valve surgery. Urine samples of 14 patients with cardiac valve diseases were collected before, during, and after CPB. The level of 8-iso-PGF was detected via selected-reaction monitoring triple quadrupole MS/MS and the result was compared with 12 healthy volunteers. The method's detection limit (3S/N) was 0.25 pg for 8-iso-PGF , with a linear working range of 0.25-20 ng/ml. For patients with cardiac valve disease, the 8-iso-PGF levels before the bypasses were the same as those of healthy individuals (P>0.05) and the 8-iso-PGF levels during and after CPB were significantly higher than those before the bypasses (P<0.05). In conclusion, we present a simple and specific protocol for LC-MS/MS quantification of urinary 8-iso-PGF collected during CPB. Using this technique, it would be feasible to assess the levels of oxidative stress during cardiac surgery and thereby helpful for the management of oxidative injury.

Original languageEnglish
Pages (from-to)237-245
Number of pages9
JournalJournal of Clinical Laboratory Analysis
Volume24
Issue number4
DOIs
StatePublished - 2010
Externally publishedYes

Keywords

  • Cardiac surgery
  • Cardiopulmonary bypass
  • Isoprostanes
  • Liquid chromatography-tandem mass spectrometry
  • Oxidative stress

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