Quantitative assessment of serum hepatitis B virus DNA: Comparison between two different methods

Chau Ting Yeh; Tse Ching Chen; Wei Chue Shyu; Rong Nan Chien; Chia Ming Chu; Yun Fan Liaw

doi:10.1016/S0928-4346(97)00037-6

Quantitative assessment of serum hepatitis B virus DNA: Comparison between two different methods

Chau Ting Yeh, Tse Ching Chen, Wei Chue Shyu, Rong Nan Chien, Chia Ming Chu, Yun Fan Liaw^*

^*Corresponding author for this work

Taipei Medical University

Research output: Contribution to journal › Journal Article › peer-review

6 Scopus citations

Abstract

Serum HBV DNA levels were quantified simultaneously by slot-blot hybridization using digoxigenin-labeled probe (S) and by Abbott HBV DNA assay (A). Samples, 69, from 64 patients seropositive for hepatitis B e antigen (HBeAg) were included and the percentages of hepatocytes containing hepatitis B core antigen (HBcAg) were also compared. The extent of HBcAg staining was only moderately correlated with HBV DNA levels detected by either Abbott (R²=0.41; P < 0.01) or slot-blot hybridization (R²=0.33; P < 0.01) assays. On the other hand, HBV DNA concentrations assessed by either method were well correlated although the levels differed markedly (S= 3.0 x A^1.31; R²=0.85; P < 0.01). These results indicated that digoxigenin-labeled probe can be used to quantify serum HBV DNA concentration, although a different range of levels was obtained when compared with Abbott HBV DNA assay.

Original language	English
Pages (from-to)	159-167
Number of pages	9
Journal	Hepatology Research
Volume	7
Issue number	3
DOIs	https://doi.org/10.1016/S0928-4346(97)00037-6
State	Published - 1997
Externally published	Yes

Keywords

DNA
Digoxigenin
Hepatitis B virus
Quantify

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/S0928-4346(97)00037-6

Cite this

@article{6a2856cb2ac442c0b6be5c5b5d4c41fb,

title = "Quantitative assessment of serum hepatitis B virus DNA: Comparison between two different methods",

abstract = "Serum HBV DNA levels were quantified simultaneously by slot-blot hybridization using digoxigenin-labeled probe (S) and by Abbott HBV DNA assay (A). Samples, 69, from 64 patients seropositive for hepatitis B e antigen (HBeAg) were included and the percentages of hepatocytes containing hepatitis B core antigen (HBcAg) were also compared. The extent of HBcAg staining was only moderately correlated with HBV DNA levels detected by either Abbott (R2=0.41; P < 0.01) or slot-blot hybridization (R2=0.33; P < 0.01) assays. On the other hand, HBV DNA concentrations assessed by either method were well correlated although the levels differed markedly (S= 3.0 x A1.31; R2=0.85; P < 0.01). These results indicated that digoxigenin-labeled probe can be used to quantify serum HBV DNA concentration, although a different range of levels was obtained when compared with Abbott HBV DNA assay.",

keywords = "DNA, Digoxigenin, Hepatitis B virus, Quantify",

author = "Yeh, \{Chau Ting\} and Chen, \{Tse Ching\} and Shyu, \{Wei Chue\} and Chien, \{Rong Nan\} and Chu, \{Chia Ming\} and Liaw, \{Yun Fan\}",

year = "1997",

doi = "10.1016/S0928-4346(97)00037-6",

language = "英语",

volume = "7",

pages = "159--167",

journal = "Hepatology Research",

issn = "1386-6346",

publisher = "Wiley-Blackwell Publishing Ltd",

number = "3",

}

TY - JOUR

T1 - Quantitative assessment of serum hepatitis B virus DNA

T2 - Comparison between two different methods

AU - Yeh, Chau Ting

AU - Chen, Tse Ching

AU - Shyu, Wei Chue

AU - Chien, Rong Nan

AU - Chu, Chia Ming

AU - Liaw, Yun Fan

PY - 1997

Y1 - 1997

N2 - Serum HBV DNA levels were quantified simultaneously by slot-blot hybridization using digoxigenin-labeled probe (S) and by Abbott HBV DNA assay (A). Samples, 69, from 64 patients seropositive for hepatitis B e antigen (HBeAg) were included and the percentages of hepatocytes containing hepatitis B core antigen (HBcAg) were also compared. The extent of HBcAg staining was only moderately correlated with HBV DNA levels detected by either Abbott (R2=0.41; P < 0.01) or slot-blot hybridization (R2=0.33; P < 0.01) assays. On the other hand, HBV DNA concentrations assessed by either method were well correlated although the levels differed markedly (S= 3.0 x A1.31; R2=0.85; P < 0.01). These results indicated that digoxigenin-labeled probe can be used to quantify serum HBV DNA concentration, although a different range of levels was obtained when compared with Abbott HBV DNA assay.

AB - Serum HBV DNA levels were quantified simultaneously by slot-blot hybridization using digoxigenin-labeled probe (S) and by Abbott HBV DNA assay (A). Samples, 69, from 64 patients seropositive for hepatitis B e antigen (HBeAg) were included and the percentages of hepatocytes containing hepatitis B core antigen (HBcAg) were also compared. The extent of HBcAg staining was only moderately correlated with HBV DNA levels detected by either Abbott (R2=0.41; P < 0.01) or slot-blot hybridization (R2=0.33; P < 0.01) assays. On the other hand, HBV DNA concentrations assessed by either method were well correlated although the levels differed markedly (S= 3.0 x A1.31; R2=0.85; P < 0.01). These results indicated that digoxigenin-labeled probe can be used to quantify serum HBV DNA concentration, although a different range of levels was obtained when compared with Abbott HBV DNA assay.

KW - DNA

KW - Digoxigenin

KW - Hepatitis B virus

KW - Quantify

UR - https://www.scopus.com/pages/publications/0030759576

U2 - 10.1016/S0928-4346(97)00037-6

DO - 10.1016/S0928-4346(97)00037-6

M3 - 文章

AN - SCOPUS:0030759576

SN - 1386-6346

VL - 7

SP - 159

EP - 167

JO - Hepatology Research

JF - Hepatology Research

IS - 3

ER -

Quantitative assessment of serum hepatitis B virus DNA: Comparison between two different methods

Abstract

Keywords

UN SDGs

Access to Document

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