Rapid and noninvasive imaging of retinal ganglion cells in live mouse models of glaucoma.

Joaquin Tosi; Nan Kai Wang; Jin Zhao; Chai Lin Chou; J. Mie Kasanuki; Stephen H. Tsang; Takayuki Nagasaki

doi:10.1007/s11307-009-0292-2

Rapid and noninvasive imaging of retinal ganglion cells in live mouse models of glaucoma.

Joaquin Tosi^*, Nan Kai Wang, Jin Zhao, Chai Lin Chou, J. Mie Kasanuki, Stephen H. Tsang, Takayuki Nagasaki

^*Corresponding author for this work

Research output: Contribution to journal › Journal Article › peer-review

8 Scopus citations

Abstract

PURPOSE: We report a noninvasive method for the monitoring of retinal ganglion cell (RGC) survival in live mice utilizing standard fluorescence microscopy. PROCEDURES: Transgenic mice expressing cyan fluorescent protein (CFP) under the regulation of an RGC-specific promoter Thy1 were used in this study. RESULTS: We established that Thy1-CFP expression is a quantitative reflection of the number of surviving RGCs, the fluorescence emission is stable for at least a year and that the loss of fluorescence correlates directly to glaucomatous damage. In high pressure glaucoma model, the peripheral retina is preferentially affected. CONCLUSIONS: Our live-imaging technique allows for the longitudinal assessment of RGC survival from the same animal. Noninvasive monitoring of neuronal cell death and survival is a powerful technique that would allow investigators to validate new potential glaucoma therapy based on neuroprotection.

Original language	English
Pages (from-to)	386-393
Number of pages	8
Journal	Molecular Imaging and Biology
Volume	12
Issue number	4
DOIs	https://doi.org/10.1007/s11307-009-0292-2
State	Published - 08 2010
Externally published	Yes

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title = "Rapid and noninvasive imaging of retinal ganglion cells in live mouse models of glaucoma.",

abstract = "PURPOSE: We report a noninvasive method for the monitoring of retinal ganglion cell (RGC) survival in live mice utilizing standard fluorescence microscopy. PROCEDURES: Transgenic mice expressing cyan fluorescent protein (CFP) under the regulation of an RGC-specific promoter Thy1 were used in this study. RESULTS: We established that Thy1-CFP expression is a quantitative reflection of the number of surviving RGCs, the fluorescence emission is stable for at least a year and that the loss of fluorescence correlates directly to glaucomatous damage. In high pressure glaucoma model, the peripheral retina is preferentially affected. CONCLUSIONS: Our live-imaging technique allows for the longitudinal assessment of RGC survival from the same animal. Noninvasive monitoring of neuronal cell death and survival is a powerful technique that would allow investigators to validate new potential glaucoma therapy based on neuroprotection.",

author = "Joaquin Tosi and Wang, {Nan Kai} and Jin Zhao and Chou, {Chai Lin} and Kasanuki, {J. Mie} and Tsang, {Stephen H.} and Takayuki Nagasaki",

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doi = "10.1007/s11307-009-0292-2",

language = "英语",

volume = "12",

pages = "386--393",

journal = "Molecular Imaging and Biology",

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TY - JOUR

T1 - Rapid and noninvasive imaging of retinal ganglion cells in live mouse models of glaucoma.

AU - Tosi, Joaquin

AU - Wang, Nan Kai

AU - Zhao, Jin

AU - Chou, Chai Lin

AU - Kasanuki, J. Mie

AU - Tsang, Stephen H.

AU - Nagasaki, Takayuki

PY - 2010/8

Y1 - 2010/8

N2 - PURPOSE: We report a noninvasive method for the monitoring of retinal ganglion cell (RGC) survival in live mice utilizing standard fluorescence microscopy. PROCEDURES: Transgenic mice expressing cyan fluorescent protein (CFP) under the regulation of an RGC-specific promoter Thy1 were used in this study. RESULTS: We established that Thy1-CFP expression is a quantitative reflection of the number of surviving RGCs, the fluorescence emission is stable for at least a year and that the loss of fluorescence correlates directly to glaucomatous damage. In high pressure glaucoma model, the peripheral retina is preferentially affected. CONCLUSIONS: Our live-imaging technique allows for the longitudinal assessment of RGC survival from the same animal. Noninvasive monitoring of neuronal cell death and survival is a powerful technique that would allow investigators to validate new potential glaucoma therapy based on neuroprotection.

AB - PURPOSE: We report a noninvasive method for the monitoring of retinal ganglion cell (RGC) survival in live mice utilizing standard fluorescence microscopy. PROCEDURES: Transgenic mice expressing cyan fluorescent protein (CFP) under the regulation of an RGC-specific promoter Thy1 were used in this study. RESULTS: We established that Thy1-CFP expression is a quantitative reflection of the number of surviving RGCs, the fluorescence emission is stable for at least a year and that the loss of fluorescence correlates directly to glaucomatous damage. In high pressure glaucoma model, the peripheral retina is preferentially affected. CONCLUSIONS: Our live-imaging technique allows for the longitudinal assessment of RGC survival from the same animal. Noninvasive monitoring of neuronal cell death and survival is a powerful technique that would allow investigators to validate new potential glaucoma therapy based on neuroprotection.

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DO - 10.1007/s11307-009-0292-2

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EP - 393

JO - Molecular Imaging and Biology

JF - Molecular Imaging and Biology

IS - 4

ER -

Rapid and noninvasive imaging of retinal ganglion cells in live mouse models of glaucoma.

Abstract

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