Rapid detection and biovar differentiation of Ureaplasma urealyticum in clinical specimens by PCR.

L. J. Teng*, S. W. Ho, H. N. Ho, S. J. Liaw, H. C. Lai, K. T. Luh

*Corresponding author for this work

Research output: Contribution to journalJournal Article peer-review

18 Scopus citations

Abstract

On the basis of the nucleotide sequence of the multiple-banded (MB) antigen genes of Ureaplasma urealyticum, a polymerase chain reaction (PCR) technique was developed for rapid detection and biovar differentiation of U. urealyticum in a total of 100 urogenital specimens from 50 female patients. Positive PCR UM-1 amplification was found in 28 cervical swabs and 31 urine samples. Overall agreement between PCR and culture was 95%. Members of the two biovars of U. urealyticum could be distinguished by the size of the PCR UM-1 amplification products. Biovar differentiation was also demonstrated by two additional sets of PCRs: PCR UM-2 and UM-3. The PCR UM-2 was used to amplify biovar 1, while PCR UM-3 amplified biovar 2 specifically. The results indicated that use of the MB antigen gene as a target for PCR amplification could provide rapid and specific detection and biotyping of ureaplasma DNA in urogenital samples.

Original languageEnglish
Pages (from-to)396-400
Number of pages5
JournalJournal of the Formosan Medical Association
Volume94
Issue number7
StatePublished - 07 1995
Externally publishedYes

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