Rapid detection of hog cholera virus in tissues by the polymerase chain reaction

Shih Tung Liu; Shui Nin Li; Ding Cheng Wang; Shu Fen Chang; Su Chuan Chiang; Wei Chuang Ho; Yu Sun Chang; Shiow Suey Lai

doi:10.1016/0166-0934(91)90138-P

Rapid detection of hog cholera virus in tissues by the polymerase chain reaction

Shih Tung Liu^*, Shui Nin Li, Ding Cheng Wang, Shu Fen Chang, Su Chuan Chiang, Wei Chuang Ho, Yu Sun Chang, Shiow Suey Lai

^*Corresponding author for this work

Research output: Contribution to journal › Journal Article › peer-review

47 Scopus citations

Abstract

A rapid method for the detection of hog cholera virus (HCV) in infected tissues, using polymerase chain reaction (PCR) was developed. Total RNA isolated from HCV-infected tissues was reverse transcribed with AMV reversé transcriptase and the resulting complementary DNA was amplified by Taq DNA polymerase in the presence of two HCV-specific primers. The amplified DNA fragment was detected by agarose gel electrophoresis. The sensitivity of this method was at 10⁴ TCID₅₀ of HCV. The sensitivity increased approximately 1000-fold when the DNA was reamplified with a set of nested primers. DNA sequencing analysis of the PCR products revealed that the HCV sequence amplified from a local field isolate was highly homologous to the HCV Alfort strain. This method may be useful for pathological and epidemiological studies of HCV in pigs.

Original language	English
Pages (from-to)	227-236
Number of pages	10
Journal	Journal of Virological Methods
Volume	35
Issue number	2
DOIs	https://doi.org/10.1016/0166-0934(91)90138-P
State	Published - 1991

Keywords

Hog cholera virus
Polymerase chain reaction

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/0166-0934(91)90138-P

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title = "Rapid detection of hog cholera virus in tissues by the polymerase chain reaction",

abstract = "A rapid method for the detection of hog cholera virus (HCV) in infected tissues, using polymerase chain reaction (PCR) was developed. Total RNA isolated from HCV-infected tissues was reverse transcribed with AMV revers{\'e} transcriptase and the resulting complementary DNA was amplified by Taq DNA polymerase in the presence of two HCV-specific primers. The amplified DNA fragment was detected by agarose gel electrophoresis. The sensitivity of this method was at 104 TCID50 of HCV. The sensitivity increased approximately 1000-fold when the DNA was reamplified with a set of nested primers. DNA sequencing analysis of the PCR products revealed that the HCV sequence amplified from a local field isolate was highly homologous to the HCV Alfort strain. This method may be useful for pathological and epidemiological studies of HCV in pigs.",

keywords = "Hog cholera virus, Polymerase chain reaction",

author = "Liu, {Shih Tung} and Li, {Shui Nin} and Wang, {Ding Cheng} and Chang, {Shu Fen} and Chiang, {Su Chuan} and Ho, {Wei Chuang} and Chang, {Yu Sun} and Lai, {Shiow Suey}",

year = "1991",

doi = "10.1016/0166-0934(91)90138-P",

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TY - JOUR

T1 - Rapid detection of hog cholera virus in tissues by the polymerase chain reaction

AU - Liu, Shih Tung

AU - Li, Shui Nin

AU - Wang, Ding Cheng

AU - Chang, Shu Fen

AU - Chiang, Su Chuan

AU - Ho, Wei Chuang

AU - Chang, Yu Sun

AU - Lai, Shiow Suey

PY - 1991

Y1 - 1991

N2 - A rapid method for the detection of hog cholera virus (HCV) in infected tissues, using polymerase chain reaction (PCR) was developed. Total RNA isolated from HCV-infected tissues was reverse transcribed with AMV reversé transcriptase and the resulting complementary DNA was amplified by Taq DNA polymerase in the presence of two HCV-specific primers. The amplified DNA fragment was detected by agarose gel electrophoresis. The sensitivity of this method was at 104 TCID50 of HCV. The sensitivity increased approximately 1000-fold when the DNA was reamplified with a set of nested primers. DNA sequencing analysis of the PCR products revealed that the HCV sequence amplified from a local field isolate was highly homologous to the HCV Alfort strain. This method may be useful for pathological and epidemiological studies of HCV in pigs.

AB - A rapid method for the detection of hog cholera virus (HCV) in infected tissues, using polymerase chain reaction (PCR) was developed. Total RNA isolated from HCV-infected tissues was reverse transcribed with AMV reversé transcriptase and the resulting complementary DNA was amplified by Taq DNA polymerase in the presence of two HCV-specific primers. The amplified DNA fragment was detected by agarose gel electrophoresis. The sensitivity of this method was at 104 TCID50 of HCV. The sensitivity increased approximately 1000-fold when the DNA was reamplified with a set of nested primers. DNA sequencing analysis of the PCR products revealed that the HCV sequence amplified from a local field isolate was highly homologous to the HCV Alfort strain. This method may be useful for pathological and epidemiological studies of HCV in pigs.

KW - Hog cholera virus

KW - Polymerase chain reaction

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U2 - 10.1016/0166-0934(91)90138-P

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EP - 236

JO - Journal of Virological Methods

JF - Journal of Virological Methods

IS - 2

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Rapid detection of hog cholera virus in tissues by the polymerase chain reaction

Abstract

Keywords

UN SDGs

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