TY - JOUR
T1 - Rapid induction of cytokine gene expression in the lung after single and fractionated doses of radiation
AU - Hong, J. H.
AU - Chiang, C. S.
AU - Tsao, C. Y.
AU - Lin, P. Y.
AU - McBride, W. H.
AU - Wu, C. J.
PY - 1999
Y1 - 1999
N2 - Purpose: To investigate cytokine gene expression in the lung after single and fractionated doses of radiation, and to investigate the effect of steroids and the genetic background. Materials and methods: Expression-of cytokine genes (mTNF-α mIL-1α, mIL-1β, mIL-2, mIL-3, mIL-4, mIL-5, mIL-6, mIFN-γ) in the lungs of C3H/HeJ and C57BL/6J mice was measured by RNase protection assay at different times after various doses of radiation. The effects of dexamethasone and fractionated radiation treatment on gene expression were also studied. Results: IL-1β was the major cytokine induced in the lungs of C3H/HeJ mice within the first day after thoracic irradiation. Radiation doses as low as 1 Gy were effective. Responses to 20Gy irradiation peaked within 4-8h and subsided by 24h. With the exception of IL-1α and TNF- α, the other cytokines that were investigated had undetectable pre-treatment mRNA levels and were not radiation inducible. Similar responses were seen in C57BL/6J mice, although TNF-α-was induced and there were some quantitative differences. Pre-treatment of C3H/HeJ mice with dexamethasone reduced basal and induced IL-1 levels, but complete inhibition was not achieved. Dexamethasone was also effective if given immediately after irradiation. Fractionated daily doses of radiation (4Gy/day) helped to maintain cytokine gene expression for a longer period. Conclusions: Inflammatory genes are rapidly induced in the lung by irradiation. This response cannot be readily abolished by steroid pre-treatment. Fractionated treatment schedules help to perpetuate the response.
AB - Purpose: To investigate cytokine gene expression in the lung after single and fractionated doses of radiation, and to investigate the effect of steroids and the genetic background. Materials and methods: Expression-of cytokine genes (mTNF-α mIL-1α, mIL-1β, mIL-2, mIL-3, mIL-4, mIL-5, mIL-6, mIFN-γ) in the lungs of C3H/HeJ and C57BL/6J mice was measured by RNase protection assay at different times after various doses of radiation. The effects of dexamethasone and fractionated radiation treatment on gene expression were also studied. Results: IL-1β was the major cytokine induced in the lungs of C3H/HeJ mice within the first day after thoracic irradiation. Radiation doses as low as 1 Gy were effective. Responses to 20Gy irradiation peaked within 4-8h and subsided by 24h. With the exception of IL-1α and TNF- α, the other cytokines that were investigated had undetectable pre-treatment mRNA levels and were not radiation inducible. Similar responses were seen in C57BL/6J mice, although TNF-α-was induced and there were some quantitative differences. Pre-treatment of C3H/HeJ mice with dexamethasone reduced basal and induced IL-1 levels, but complete inhibition was not achieved. Dexamethasone was also effective if given immediately after irradiation. Fractionated daily doses of radiation (4Gy/day) helped to maintain cytokine gene expression for a longer period. Conclusions: Inflammatory genes are rapidly induced in the lung by irradiation. This response cannot be readily abolished by steroid pre-treatment. Fractionated treatment schedules help to perpetuate the response.
UR - http://www.scopus.com/inward/record.url?scp=0032726778&partnerID=8YFLogxK
U2 - 10.1080/095530099139287
DO - 10.1080/095530099139287
M3 - 文章
C2 - 10597915
AN - SCOPUS:0032726778
SN - 0955-3002
VL - 75
SP - 1421
EP - 1427
JO - International Journal of Radiation Biology
JF - International Journal of Radiation Biology
IS - 11
ER -