Real-Time and Non-invasive Measurement of 3D Cancer Cell Invasion Process under IL-6 Cytokine Stimulation

Chun Hao Huang; Kin Fong Lei

doi:10.1109/NANOMED49242.2019.9130623

Real-Time and Non-invasive Measurement of 3D Cancer Cell Invasion Process under IL-6 Cytokine Stimulation

Chun Hao Huang
, Kin Fong Lei^*

^*Corresponding author for this work

Master Program in Nano-Electronic Engineering and Design

Chang Gung University
Chang Gung Memorial Hospital

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

Abstract

Cancer cells possess a broad spectrum of invasion mechanisms. Quantitative analysis of cancer cell invasion process under tested condition is important to precisely study the cellular invasion capability. In this study, a microfluidic device was developed and electrodes were embedded in the microchannel for the impedimetric measurement of cell invasion. Cancer cells were stimulated by interleukin-6 cytokine and invaded along the hydrogel-filled microchannel. The three-dimensional (3D) cell invasion process was monitored by measuring the impedance across the electrodes. The cell invasion speed could be calculated based on the cell invasion distance divided by time. Thus, 3D cell invasion process was demonstrated to be quantitatively monitored in real-time and non-invasive manner. The current development provides a promising and quantitative tool for cell invasion assay.

Original language	English
Title of host publication	2019 IEEE 13th International Conference on Nano/Molecular Medicine and Engineering, NANOMED 2019
Publisher	IEEE Computer Society
Pages	26-29
Number of pages	4
ISBN (Electronic)	9781728166483
DOIs	https://doi.org/10.1109/NANOMED49242.2019.9130623
State	Published - 11 2019
Event	13th IEEE International Conference on Nano/Molecular Medicine and Engineering, NANOMED 2019 - Gwangju, Korea, Republic of Duration: 21 11 2019 → 24 11 2019

Publication series

Name	IEEE International Conference on Nano/Molecular Medicine and Engineering, NANOMED
Volume	2020-November
ISSN (Print)	2159-6964
ISSN (Electronic)	2159-6972

Conference

Conference	13th IEEE International Conference on Nano/Molecular Medicine and Engineering, NANOMED 2019
Country/Territory	Korea, Republic of
City	Gwangju
Period	21/11/19 → 24/11/19

Bibliographical note

Publisher Copyright:
© 2019 IEEE.

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

Cell invasion
Cytokine stimulation
Impedance measurement
Microfluidic system

Access to Document

10.1109/NANOMED49242.2019.9130623

Cite this

Huang, C. H., & Lei, K. F. (2019). Real-Time and Non-invasive Measurement of 3D Cancer Cell Invasion Process under IL-6 Cytokine Stimulation. In 2019 IEEE 13th International Conference on Nano/Molecular Medicine and Engineering, NANOMED 2019 (pp. 26-29). Article 9130623 (IEEE International Conference on Nano/Molecular Medicine and Engineering, NANOMED; Vol. 2020-November). IEEE Computer Society. https://doi.org/10.1109/NANOMED49242.2019.9130623

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title = "Real-Time and Non-invasive Measurement of 3D Cancer Cell Invasion Process under IL-6 Cytokine Stimulation",

abstract = "Cancer cells possess a broad spectrum of invasion mechanisms. Quantitative analysis of cancer cell invasion process under tested condition is important to precisely study the cellular invasion capability. In this study, a microfluidic device was developed and electrodes were embedded in the microchannel for the impedimetric measurement of cell invasion. Cancer cells were stimulated by interleukin-6 cytokine and invaded along the hydrogel-filled microchannel. The three-dimensional (3D) cell invasion process was monitored by measuring the impedance across the electrodes. The cell invasion speed could be calculated based on the cell invasion distance divided by time. Thus, 3D cell invasion process was demonstrated to be quantitatively monitored in real-time and non-invasive manner. The current development provides a promising and quantitative tool for cell invasion assay.",

keywords = "Cell invasion, Cytokine stimulation, Impedance measurement, Microfluidic system",

author = "Huang, \{Chun Hao\} and Lei, \{Kin Fong\}",

note = "Publisher Copyright: {\textcopyright} 2019 IEEE.; 13th IEEE International Conference on Nano/Molecular Medicine and Engineering, NANOMED 2019 ; Conference date: 21-11-2019 Through 24-11-2019",

year = "2019",

month = nov,

doi = "10.1109/NANOMED49242.2019.9130623",

language = "英语",

series = "IEEE International Conference on Nano/Molecular Medicine and Engineering, NANOMED",

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Huang, CH & Lei, KF 2019, Real-Time and Non-invasive Measurement of 3D Cancer Cell Invasion Process under IL-6 Cytokine Stimulation. in 2019 IEEE 13th International Conference on Nano/Molecular Medicine and Engineering, NANOMED 2019., 9130623, IEEE International Conference on Nano/Molecular Medicine and Engineering, NANOMED, vol. 2020-November, IEEE Computer Society, pp. 26-29, 13th IEEE International Conference on Nano/Molecular Medicine and Engineering, NANOMED 2019, Gwangju, Korea, Republic of, 21/11/19. https://doi.org/10.1109/NANOMED49242.2019.9130623

Real-Time and Non-invasive Measurement of 3D Cancer Cell Invasion Process under IL-6 Cytokine Stimulation. / Huang, Chun Hao; Lei, Kin Fong.
2019 IEEE 13th International Conference on Nano/Molecular Medicine and Engineering, NANOMED 2019. IEEE Computer Society, 2019. p. 26-29 9130623 (IEEE International Conference on Nano/Molecular Medicine and Engineering, NANOMED; Vol. 2020-November).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

TY - GEN

T1 - Real-Time and Non-invasive Measurement of 3D Cancer Cell Invasion Process under IL-6 Cytokine Stimulation

AU - Huang, Chun Hao

AU - Lei, Kin Fong

PY - 2019/11

Y1 - 2019/11

N2 - Cancer cells possess a broad spectrum of invasion mechanisms. Quantitative analysis of cancer cell invasion process under tested condition is important to precisely study the cellular invasion capability. In this study, a microfluidic device was developed and electrodes were embedded in the microchannel for the impedimetric measurement of cell invasion. Cancer cells were stimulated by interleukin-6 cytokine and invaded along the hydrogel-filled microchannel. The three-dimensional (3D) cell invasion process was monitored by measuring the impedance across the electrodes. The cell invasion speed could be calculated based on the cell invasion distance divided by time. Thus, 3D cell invasion process was demonstrated to be quantitatively monitored in real-time and non-invasive manner. The current development provides a promising and quantitative tool for cell invasion assay.

AB - Cancer cells possess a broad spectrum of invasion mechanisms. Quantitative analysis of cancer cell invasion process under tested condition is important to precisely study the cellular invasion capability. In this study, a microfluidic device was developed and electrodes were embedded in the microchannel for the impedimetric measurement of cell invasion. Cancer cells were stimulated by interleukin-6 cytokine and invaded along the hydrogel-filled microchannel. The three-dimensional (3D) cell invasion process was monitored by measuring the impedance across the electrodes. The cell invasion speed could be calculated based on the cell invasion distance divided by time. Thus, 3D cell invasion process was demonstrated to be quantitatively monitored in real-time and non-invasive manner. The current development provides a promising and quantitative tool for cell invasion assay.

KW - Cell invasion

KW - Cytokine stimulation

KW - Impedance measurement

KW - Microfluidic system

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DO - 10.1109/NANOMED49242.2019.9130623

M3 - 会议稿件

AN - SCOPUS:85088321262

T3 - IEEE International Conference on Nano/Molecular Medicine and Engineering, NANOMED

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BT - 2019 IEEE 13th International Conference on Nano/Molecular Medicine and Engineering, NANOMED 2019

PB - IEEE Computer Society

T2 - 13th IEEE International Conference on Nano/Molecular Medicine and Engineering, NANOMED 2019

Y2 - 21 November 2019 through 24 November 2019

ER -

Huang CH, Lei KF. Real-Time and Non-invasive Measurement of 3D Cancer Cell Invasion Process under IL-6 Cytokine Stimulation. In 2019 IEEE 13th International Conference on Nano/Molecular Medicine and Engineering, NANOMED 2019. IEEE Computer Society. 2019. p. 26-29. 9130623. (IEEE International Conference on Nano/Molecular Medicine and Engineering, NANOMED). doi: 10.1109/NANOMED49242.2019.9130623

Real-Time and Non-invasive Measurement of 3D Cancer Cell Invasion Process under IL-6 Cytokine Stimulation

Abstract

Publication series

Conference

Bibliographical note

UN SDGs

Keywords

Access to Document

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