Real-time PCR analysis of the intestinal microbiotas in peritoneal dialysis patients

I. Kuan Wang; Hsueh Chou Lai; Cheng Ju Yu; Chih Chia Liang; Chiz Tzung Chang; Huey Liang Kuo; Ya Fei Yang; Chung Chih Lin; Hsin Hung Lin; Yao Lung Liu; Yi Chih Chang; Yi Ying Wu; Chu Huang Chen; Chi Yuan Li; Feng Rong Chuang; Chiu Ching Huang; Chih Hsueh Lin; Hung Chih Lin

doi:10.1128/AEM.05605-11

Real-time PCR analysis of the intestinal microbiotas in peritoneal dialysis patients

I. Kuan Wang, Hsueh Chou Lai, Cheng Ju Yu, Chih Chia Liang, Chiz Tzung Chang, Huey Liang Kuo, Ya Fei Yang, Chung Chih Lin, Hsin Hung Lin, Yao Lung Liu, Yi Chih Chang, Yi Ying Wu, Chu Huang Chen, Chi Yuan Li, Feng Rong Chuang, Chiu Ching Huang, Chih Hsueh Lin, Hung Chih Lin^*

^*Corresponding author for this work

Research output: Contribution to journal › Journal Article › peer-review

106 Scopus citations

Abstract

Bifidobacterium and Lactobacillus can beneficially affect the host by producing acetic acid and lactic acid, which lower pH and thereby inhibit the growth of pathogens or allow the probiotic bacteria to compete with pathogens for epithelial adhesion sites and nutrients. The transmural migration of enteric organisms into the peritoneal cavity can cause peritonitis in peritoneal dialysis (PD) patients. We hypothesized that the composition of the intestinal microbiota with regard to Lactobacillus species and Bifidobacterium species differed between PD patients and healthy controls. The aim of the study was to investigate these differences by real-time PCR analysis of fecal samples. From 1 August 2009 to 31 March 2010, a total of 29 nondiabetic PD patients and 41 healthy controls from China Medical University Hospital were recruited after giving their informed consent. Fecal samples were collected from the PD patients and their age-matched counterparts in the morning using a standardized procedure. DNA extracted from these samples was analyzed by real-time PCR. All bifidobacteria, Bifidobacterium catenulatum, B. longum, B. bifidum, Lactobacillus plantarum, L. paracasei, and Klebsiella pneumoniae were less frequently detected in the patient samples. Dysbiosis (microbial imbalance) may impair intestinal barrier function and increase host vulnerability to pathogen invasion. Further studies are necessary to confirm our findings before clinical trials with probiotic supplementation in PD patients.

Original language	English
Pages (from-to)	1107-1112
Number of pages	6
Journal	Applied and Environmental Microbiology
Volume	78
Issue number	4
DOIs	https://doi.org/10.1128/AEM.05605-11
State	Published - 02 2012
Externally published	Yes

Access to Document

10.1128/AEM.05605-11

Cite this

Wang, I. K., Lai, H. C., Yu, C. J., Liang, C. C., Chang, C. T., Kuo, H. L., Yang, Y. F., Lin, C. C., Lin, H. H., Liu, Y. L., Chang, Y. C., Wu, Y. Y., Chen, C. H., Li, C. Y., Chuang, F. R., Huang, C. C., Lin, C. H., & Lin, H. C. (2012). Real-time PCR analysis of the intestinal microbiotas in peritoneal dialysis patients. Applied and Environmental Microbiology, 78(4), 1107-1112. https://doi.org/10.1128/AEM.05605-11

@article{135334a3169a42bda03d73f8f2fb1fef,

title = "Real-time PCR analysis of the intestinal microbiotas in peritoneal dialysis patients",

abstract = "Bifidobacterium and Lactobacillus can beneficially affect the host by producing acetic acid and lactic acid, which lower pH and thereby inhibit the growth of pathogens or allow the probiotic bacteria to compete with pathogens for epithelial adhesion sites and nutrients. The transmural migration of enteric organisms into the peritoneal cavity can cause peritonitis in peritoneal dialysis (PD) patients. We hypothesized that the composition of the intestinal microbiota with regard to Lactobacillus species and Bifidobacterium species differed between PD patients and healthy controls. The aim of the study was to investigate these differences by real-time PCR analysis of fecal samples. From 1 August 2009 to 31 March 2010, a total of 29 nondiabetic PD patients and 41 healthy controls from China Medical University Hospital were recruited after giving their informed consent. Fecal samples were collected from the PD patients and their age-matched counterparts in the morning using a standardized procedure. DNA extracted from these samples was analyzed by real-time PCR. All bifidobacteria, Bifidobacterium catenulatum, B. longum, B. bifidum, Lactobacillus plantarum, L. paracasei, and Klebsiella pneumoniae were less frequently detected in the patient samples. Dysbiosis (microbial imbalance) may impair intestinal barrier function and increase host vulnerability to pathogen invasion. Further studies are necessary to confirm our findings before clinical trials with probiotic supplementation in PD patients.",

author = "Wang, {I. Kuan} and Lai, {Hsueh Chou} and Yu, {Cheng Ju} and Liang, {Chih Chia} and Chang, {Chiz Tzung} and Kuo, {Huey Liang} and Yang, {Ya Fei} and Lin, {Chung Chih} and Lin, {Hsin Hung} and Liu, {Yao Lung} and Chang, {Yi Chih} and Wu, {Yi Ying} and Chen, {Chu Huang} and Li, {Chi Yuan} and Chuang, {Feng Rong} and Huang, {Chiu Ching} and Lin, {Chih Hsueh} and Lin, {Hung Chih}",

year = "2012",

month = feb,

doi = "10.1128/AEM.05605-11",

language = "英语",

volume = "78",

pages = "1107--1112",

journal = "Applied and Environmental Microbiology",

issn = "0099-2240",

publisher = "American Society for Microbiology",

number = "4",

}

Wang, IK, Lai, HC, Yu, CJ, Liang, CC, Chang, CT, Kuo, HL, Yang, YF, Lin, CC, Lin, HH, Liu, YL, Chang, YC, Wu, YY, Chen, CH, Li, CY, Chuang, FR, Huang, CC, Lin, CH & Lin, HC 2012, 'Real-time PCR analysis of the intestinal microbiotas in peritoneal dialysis patients', Applied and Environmental Microbiology, vol. 78, no. 4, pp. 1107-1112. https://doi.org/10.1128/AEM.05605-11

TY - JOUR

T1 - Real-time PCR analysis of the intestinal microbiotas in peritoneal dialysis patients

AU - Wang, I. Kuan

AU - Lai, Hsueh Chou

AU - Yu, Cheng Ju

AU - Liang, Chih Chia

AU - Chang, Chiz Tzung

AU - Kuo, Huey Liang

AU - Yang, Ya Fei

AU - Lin, Chung Chih

AU - Lin, Hsin Hung

AU - Liu, Yao Lung

AU - Chang, Yi Chih

AU - Wu, Yi Ying

AU - Chen, Chu Huang

AU - Li, Chi Yuan

AU - Chuang, Feng Rong

AU - Huang, Chiu Ching

AU - Lin, Chih Hsueh

AU - Lin, Hung Chih

PY - 2012/2

Y1 - 2012/2

N2 - Bifidobacterium and Lactobacillus can beneficially affect the host by producing acetic acid and lactic acid, which lower pH and thereby inhibit the growth of pathogens or allow the probiotic bacteria to compete with pathogens for epithelial adhesion sites and nutrients. The transmural migration of enteric organisms into the peritoneal cavity can cause peritonitis in peritoneal dialysis (PD) patients. We hypothesized that the composition of the intestinal microbiota with regard to Lactobacillus species and Bifidobacterium species differed between PD patients and healthy controls. The aim of the study was to investigate these differences by real-time PCR analysis of fecal samples. From 1 August 2009 to 31 March 2010, a total of 29 nondiabetic PD patients and 41 healthy controls from China Medical University Hospital were recruited after giving their informed consent. Fecal samples were collected from the PD patients and their age-matched counterparts in the morning using a standardized procedure. DNA extracted from these samples was analyzed by real-time PCR. All bifidobacteria, Bifidobacterium catenulatum, B. longum, B. bifidum, Lactobacillus plantarum, L. paracasei, and Klebsiella pneumoniae were less frequently detected in the patient samples. Dysbiosis (microbial imbalance) may impair intestinal barrier function and increase host vulnerability to pathogen invasion. Further studies are necessary to confirm our findings before clinical trials with probiotic supplementation in PD patients.

AB - Bifidobacterium and Lactobacillus can beneficially affect the host by producing acetic acid and lactic acid, which lower pH and thereby inhibit the growth of pathogens or allow the probiotic bacteria to compete with pathogens for epithelial adhesion sites and nutrients. The transmural migration of enteric organisms into the peritoneal cavity can cause peritonitis in peritoneal dialysis (PD) patients. We hypothesized that the composition of the intestinal microbiota with regard to Lactobacillus species and Bifidobacterium species differed between PD patients and healthy controls. The aim of the study was to investigate these differences by real-time PCR analysis of fecal samples. From 1 August 2009 to 31 March 2010, a total of 29 nondiabetic PD patients and 41 healthy controls from China Medical University Hospital were recruited after giving their informed consent. Fecal samples were collected from the PD patients and their age-matched counterparts in the morning using a standardized procedure. DNA extracted from these samples was analyzed by real-time PCR. All bifidobacteria, Bifidobacterium catenulatum, B. longum, B. bifidum, Lactobacillus plantarum, L. paracasei, and Klebsiella pneumoniae were less frequently detected in the patient samples. Dysbiosis (microbial imbalance) may impair intestinal barrier function and increase host vulnerability to pathogen invasion. Further studies are necessary to confirm our findings before clinical trials with probiotic supplementation in PD patients.

UR - http://www.scopus.com/inward/record.url?scp=84863116056&partnerID=8YFLogxK

U2 - 10.1128/AEM.05605-11

DO - 10.1128/AEM.05605-11

M3 - 文章

C2 - 22179250

AN - SCOPUS:84863116056

SN - 0099-2240

VL - 78

SP - 1107

EP - 1112

JO - Applied and Environmental Microbiology

JF - Applied and Environmental Microbiology

IS - 4

ER -

Real-time PCR analysis of the intestinal microbiotas in peritoneal dialysis patients

Abstract

Access to Document

Other files and links

Fingerprint

Cite this