Recombinant lipidated FLIPr effectively enhances mucosal and systemic immune responses for various vaccine types

Ming Shu Hsieh; Mei Yu Chen; Chia Wei Hsu; Yu Wen Tsai; Fang Feng Chiu; Cheng Lung Hsu; Chang Ling Lin; Chiao Chieh Wu; Ling Ling Tu; Chen Yi Chiang; Shih Jen Liu; Ching Len Liao; Hsin Wei Chen

doi:10.1038/s41541-023-00680-4

Recombinant lipidated FLIPr effectively enhances mucosal and systemic immune responses for various vaccine types

Ming Shu Hsieh, Mei Yu Chen, Chia Wei Hsu, Yu Wen Tsai, Fang Feng Chiu, Cheng Lung Hsu, Chang Ling Lin, Chiao Chieh Wu, Ling Ling Tu, Chen Yi Chiang, Shih Jen Liu, Ching Len Liao, Hsin Wei Chen^*

^*Corresponding author for this work

School of Medicine

Research output: Contribution to journal › Journal Article › peer-review

3 Scopus citations

Abstract

Formyl peptide receptor-like 1 inhibitor protein (FLIPr) is an immune evasion protein produced by Staphylococcus aureus, and FLIPr is a potential vaccine candidate for reducing Staphylococcus aureus virulence and biofilm formation. We produced recombinant lipidated FLIPr (rLF) to increase the immunogenicity of FLIPr and showed that rLF alone elicited potent anti-FLIPr antibody responses to overcome the FLIPr-mediated inhibition of phagocytosis. In addition, rLF has potent immunostimulatory properties. We demonstrated that rLF is an effective adjuvant. When an antigen is formulated with rLF, it can induce long-lasting antigen-specific immune responses and enhance mucosal and systemic antibody responses as well as broad-spectrum T-cell responses in mice. These findings support further exploration of rLF in the clinic as an adjuvant for various vaccine types with extra benefits to abolish FLIPr-mediated immunosuppressive effects.

Original language	English
Article number	82
Pages (from-to)	82
Journal	npj Vaccines
Volume	8
Issue number	1
DOIs	https://doi.org/10.1038/s41541-023-00680-4
State	Published - 02 06 2023

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title = "Recombinant lipidated FLIPr effectively enhances mucosal and systemic immune responses for various vaccine types",

abstract = "Formyl peptide receptor-like 1 inhibitor protein (FLIPr) is an immune evasion protein produced by Staphylococcus aureus, and FLIPr is a potential vaccine candidate for reducing Staphylococcus aureus virulence and biofilm formation. We produced recombinant lipidated FLIPr (rLF) to increase the immunogenicity of FLIPr and showed that rLF alone elicited potent anti-FLIPr antibody responses to overcome the FLIPr-mediated inhibition of phagocytosis. In addition, rLF has potent immunostimulatory properties. We demonstrated that rLF is an effective adjuvant. When an antigen is formulated with rLF, it can induce long-lasting antigen-specific immune responses and enhance mucosal and systemic antibody responses as well as broad-spectrum T-cell responses in mice. These findings support further exploration of rLF in the clinic as an adjuvant for various vaccine types with extra benefits to abolish FLIPr-mediated immunosuppressive effects.",

author = "Hsieh, {Ming Shu} and Chen, {Mei Yu} and Hsu, {Chia Wei} and Tsai, {Yu Wen} and Chiu, {Fang Feng} and Hsu, {Cheng Lung} and Lin, {Chang Ling} and Wu, {Chiao Chieh} and Tu, {Ling Ling} and Chiang, {Chen Yi} and Liu, {Shih Jen} and Liao, {Ching Len} and Chen, {Hsin Wei}",

note = "{\textcopyright} 2023. The Author(s).",

year = "2023",

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doi = "10.1038/s41541-023-00680-4",

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T1 - Recombinant lipidated FLIPr effectively enhances mucosal and systemic immune responses for various vaccine types

AU - Hsieh, Ming Shu

AU - Chen, Mei Yu

AU - Hsu, Chia Wei

AU - Tsai, Yu Wen

AU - Chiu, Fang Feng

AU - Hsu, Cheng Lung

AU - Lin, Chang Ling

AU - Wu, Chiao Chieh

AU - Tu, Ling Ling

AU - Chiang, Chen Yi

AU - Liu, Shih Jen

AU - Liao, Ching Len

AU - Chen, Hsin Wei

PY - 2023/6/2

Y1 - 2023/6/2

N2 - Formyl peptide receptor-like 1 inhibitor protein (FLIPr) is an immune evasion protein produced by Staphylococcus aureus, and FLIPr is a potential vaccine candidate for reducing Staphylococcus aureus virulence and biofilm formation. We produced recombinant lipidated FLIPr (rLF) to increase the immunogenicity of FLIPr and showed that rLF alone elicited potent anti-FLIPr antibody responses to overcome the FLIPr-mediated inhibition of phagocytosis. In addition, rLF has potent immunostimulatory properties. We demonstrated that rLF is an effective adjuvant. When an antigen is formulated with rLF, it can induce long-lasting antigen-specific immune responses and enhance mucosal and systemic antibody responses as well as broad-spectrum T-cell responses in mice. These findings support further exploration of rLF in the clinic as an adjuvant for various vaccine types with extra benefits to abolish FLIPr-mediated immunosuppressive effects.

AB - Formyl peptide receptor-like 1 inhibitor protein (FLIPr) is an immune evasion protein produced by Staphylococcus aureus, and FLIPr is a potential vaccine candidate for reducing Staphylococcus aureus virulence and biofilm formation. We produced recombinant lipidated FLIPr (rLF) to increase the immunogenicity of FLIPr and showed that rLF alone elicited potent anti-FLIPr antibody responses to overcome the FLIPr-mediated inhibition of phagocytosis. In addition, rLF has potent immunostimulatory properties. We demonstrated that rLF is an effective adjuvant. When an antigen is formulated with rLF, it can induce long-lasting antigen-specific immune responses and enhance mucosal and systemic antibody responses as well as broad-spectrum T-cell responses in mice. These findings support further exploration of rLF in the clinic as an adjuvant for various vaccine types with extra benefits to abolish FLIPr-mediated immunosuppressive effects.

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AN - SCOPUS:85160925840

SN - 2059-0105

VL - 8

SP - 82

JO - npj Vaccines

JF - npj Vaccines

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M1 - 82

ER -

Recombinant lipidated FLIPr effectively enhances mucosal and systemic immune responses for various vaccine types

Abstract

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