Recovery of yeast invertase from ethanol fermentation broth

Err cheng Chan*, Cheeshan S. Chen, Li Fu Chen

*Corresponding author for this work

Research output: Contribution to journalJournal Article peer-review

2 Scopus citations


Ethanol fermentation broth produced by an aggregated form of Saccharomycesuvarum strain contained invertase when sucrose-based raw materials were used. The amount of invertase in the borth was in the range of 1.4 to 4.8 units/ml, which was affected by the dilution rate, the concentration of corn steep liquor, and the type of sugar used. The activity of invertase in the broth could be maintained at 0.8 units/ml over two months. When the broth was passed through DEAE-cellulose beads and eluted with a NaCl-Tris-HCl buffer solution, a 75% recovery yield of invertase with 9-fold purification and 30-fold concentration could be achieved.

Original languageEnglish
Pages (from-to)573-576
Number of pages4
JournalBiotechnology Letters
Issue number7
StatePublished - 07 1992
Externally publishedYes


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