Reduced nicotinamide adenine dinucleotide fluorescence lifetime separates human mesenchymal stem cells from differentiated progenies

Han Wen Guo; Chien Tsun Chen; Yau Huei Wei; Oscar K. Lee; Vladimir Gukassyan; Fu Jen Kao; Hsing Wen Wang

doi:10.1117/1.2990752

Reduced nicotinamide adenine dinucleotide fluorescence lifetime separates human mesenchymal stem cells from differentiated progenies

Han Wen Guo, Chien Tsun Chen, Yau Huei Wei, Oscar K. Lee, Vladimir Gukassyan, Fu Jen Kao, Hsing Wen Wang^*

^*Corresponding author for this work

National Yang Ming Chiao Tung University

Research output: Contribution to journal › Journal Article › peer-review

52 Scopus citations

Abstract

The metabolic changes of human mesenchymal stem cells (hMSCs) during osteogenic differentiation were accessed by reduced nicotinamide adenine dinucleotide (NADH) fluorescence lifetime. An increase in mean fluorescence lifetime and decrease in the ratio between free NADH and protein-bound NADH correlated with our previously reported increase in the adenosine triphosphate (ATP) level of hMSCs during differentiation. These findings suggest that NADH fluorescence lifetime may serve as a new optical biomarker for noninvasive selection of stem cells from differentiated progenies.

Original language	English
Article number	050505
Journal	Journal of Biomedical Optics
Volume	13
Issue number	5
DOIs	https://doi.org/10.1117/1.2990752
State	Published - 2008
Externally published	Yes

Keywords

fluorescence lifetime
microscopy
stem cell

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10.1117/1.2990752

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title = "Reduced nicotinamide adenine dinucleotide fluorescence lifetime separates human mesenchymal stem cells from differentiated progenies",

abstract = "The metabolic changes of human mesenchymal stem cells (hMSCs) during osteogenic differentiation were accessed by reduced nicotinamide adenine dinucleotide (NADH) fluorescence lifetime. An increase in mean fluorescence lifetime and decrease in the ratio between free NADH and protein-bound NADH correlated with our previously reported increase in the adenosine triphosphate (ATP) level of hMSCs during differentiation. These findings suggest that NADH fluorescence lifetime may serve as a new optical biomarker for noninvasive selection of stem cells from differentiated progenies.",

keywords = "fluorescence lifetime, microscopy, stem cell",

author = "Guo, {Han Wen} and Chen, {Chien Tsun} and Wei, {Yau Huei} and Lee, {Oscar K.} and Vladimir Gukassyan and Kao, {Fu Jen} and Wang, {Hsing Wen}",

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T1 - Reduced nicotinamide adenine dinucleotide fluorescence lifetime separates human mesenchymal stem cells from differentiated progenies

AU - Guo, Han Wen

AU - Chen, Chien Tsun

AU - Wei, Yau Huei

AU - Lee, Oscar K.

AU - Gukassyan, Vladimir

AU - Kao, Fu Jen

AU - Wang, Hsing Wen

PY - 2008

Y1 - 2008

N2 - The metabolic changes of human mesenchymal stem cells (hMSCs) during osteogenic differentiation were accessed by reduced nicotinamide adenine dinucleotide (NADH) fluorescence lifetime. An increase in mean fluorescence lifetime and decrease in the ratio between free NADH and protein-bound NADH correlated with our previously reported increase in the adenosine triphosphate (ATP) level of hMSCs during differentiation. These findings suggest that NADH fluorescence lifetime may serve as a new optical biomarker for noninvasive selection of stem cells from differentiated progenies.

AB - The metabolic changes of human mesenchymal stem cells (hMSCs) during osteogenic differentiation were accessed by reduced nicotinamide adenine dinucleotide (NADH) fluorescence lifetime. An increase in mean fluorescence lifetime and decrease in the ratio between free NADH and protein-bound NADH correlated with our previously reported increase in the adenosine triphosphate (ATP) level of hMSCs during differentiation. These findings suggest that NADH fluorescence lifetime may serve as a new optical biomarker for noninvasive selection of stem cells from differentiated progenies.

KW - fluorescence lifetime

KW - microscopy

KW - stem cell

UR - http://www.scopus.com/inward/record.url?scp=60849084594&partnerID=8YFLogxK

U2 - 10.1117/1.2990752

DO - 10.1117/1.2990752

M3 - 文章

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AN - SCOPUS:60849084594

SN - 1083-3668

VL - 13

JO - Journal of Biomedical Optics

JF - Journal of Biomedical Optics

IS - 5

M1 - 050505

ER -

Reduced nicotinamide adenine dinucleotide fluorescence lifetime separates human mesenchymal stem cells from differentiated progenies

Abstract

Keywords

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