Regulation of thyroid hormones on the production of testosterone in rats

Yu Chung Chiao; Hsien Yang Lee; Shyi Wu Wang; Jiuan Jiuan Hwang; Chau Heng Chien; Seng Wong Huang; Chien Chen Lu; Jiann Jong Chen; Shiow Chwen Tsai; Paulus S. Wang

doi:10.1002/(SICI)1097-4644(19990615)73:4<554::AID-JCB13>3.0.CO;2-L

Regulation of thyroid hormones on the production of testosterone in rats

Yu Chung Chiao
, Hsien Yang Lee
, Shyi Wu Wang
, Jiuan Jiuan Hwang
, Chau Heng Chien
, Seng Wong Huang
, Chien Chen Lu
, Jiann Jong Chen
, Shiow Chwen Tsai
, Paulus S. Wang^*

^*Corresponding author for this work

Department of Physiology

Research output: Contribution to journal › Journal Article › peer-review

26 Scopus citations

Abstract

The effects of a thyroidectomy and thyroxine (T₄) replacement on the spontaneous and human chorionic gonadotropin (hCG)-stimulated secretion of testosterone and the production of adenosine 3',5'-cyclic monophosphate (cAMP) in rat testes were studied. Thyroidectomy decreased the basal levels of plasma luteinizing hormone (LH) and testosterone, which delayed the maximal response of testosterone to gonadotropin-releasing hormone (GnRH) and hCG in male rats. T₄ replacement in thyroparathyroidectomized (Tx) rats restored the concentrations of plasma LH and testosterone to euthyroid levels. Thyroidectomy decreased the basal release of hypothalamic GnRH, pituitary LH, and testicular testosterone as well as the LH response to GnRH and testosterone response to hCG in vitro. T₄ replacement in Tx rats restored the in vitro release of GnRH, GnRH-stimulated LH release as well as hCG-stimulated testosterone release. Administration of T₄ in vitro restored the release of testosterone by rat testicular interstitial cells (TICs). The increase of testosterone release in response to forskolin and androstenedione was less in TICs from Tx rats than in that from sham Tx rats. Administration of nifedipine in vitro resulted in a decrease of testosterone release by TICs from sham Tx but not from Tx rats. The basal level of cAMP in TICs was decreased by thyroidectomy. The increased accumulation of cAMP in TICs following administration of forskolin was eliminated in Tx rats. T₄ replacement in Tx restored the testosterone response to forskolin. But the testosterone response to androstenedione and the cAMP response to forskolin in TICs was not restored by T₄ in Tx rats. These results suggest that the inhibitory effect of a thyroidectomy on the production of testosterone in rat TICs is in part due to: 1) the decreased basal secretion of pituitary LH and its response to GnRH; 2) the decreased response of TICs to gonadotropin; and 3) the diminished production of cAMP, influx of calcium, and activity of 17β-HSD. T₄ may enhance testosterone production by acting directly at the testicular interstitial cells of Tx rats.

Original language	English
Pages (from-to)	554-562
Number of pages	9
Journal	Journal of Cellular Biochemistry
Volume	73
Issue number	4
DOIs	https://doi.org/10.1002/(SICI)1097-4644(19990615)73:4<554::AID-JCB13>3.0.CO;2-L
State	Published - 15 06 1999

Keywords

GnRH
LH
T
T
Tx
cAMP

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10.1002/(SICI)1097-4644(19990615)73:4<554::AID-JCB13>3.0.CO;2-L

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@article{3f0cd4001e1f48768469a52aeb82cdf1,

title = "Regulation of thyroid hormones on the production of testosterone in rats",

abstract = "The effects of a thyroidectomy and thyroxine (T4) replacement on the spontaneous and human chorionic gonadotropin (hCG)-stimulated secretion of testosterone and the production of adenosine 3',5'-cyclic monophosphate (cAMP) in rat testes were studied. Thyroidectomy decreased the basal levels of plasma luteinizing hormone (LH) and testosterone, which delayed the maximal response of testosterone to gonadotropin-releasing hormone (GnRH) and hCG in male rats. T4 replacement in thyroparathyroidectomized (Tx) rats restored the concentrations of plasma LH and testosterone to euthyroid levels. Thyroidectomy decreased the basal release of hypothalamic GnRH, pituitary LH, and testicular testosterone as well as the LH response to GnRH and testosterone response to hCG in vitro. T4 replacement in Tx rats restored the in vitro release of GnRH, GnRH-stimulated LH release as well as hCG-stimulated testosterone release. Administration of T4 in vitro restored the release of testosterone by rat testicular interstitial cells (TICs). The increase of testosterone release in response to forskolin and androstenedione was less in TICs from Tx rats than in that from sham Tx rats. Administration of nifedipine in vitro resulted in a decrease of testosterone release by TICs from sham Tx but not from Tx rats. The basal level of cAMP in TICs was decreased by thyroidectomy. The increased accumulation of cAMP in TICs following administration of forskolin was eliminated in Tx rats. T4 replacement in Tx restored the testosterone response to forskolin. But the testosterone response to androstenedione and the cAMP response to forskolin in TICs was not restored by T4 in Tx rats. These results suggest that the inhibitory effect of a thyroidectomy on the production of testosterone in rat TICs is in part due to: 1) the decreased basal secretion of pituitary LH and its response to GnRH; 2) the decreased response of TICs to gonadotropin; and 3) the diminished production of cAMP, influx of calcium, and activity of 17β-HSD. T4 may enhance testosterone production by acting directly at the testicular interstitial cells of Tx rats.",

keywords = "GnRH, LH, T, T, Tx, cAMP",

author = "Chiao, \{Yu Chung\} and Lee, \{Hsien Yang\} and Wang, \{Shyi Wu\} and Hwang, \{Jiuan Jiuan\} and Chien, \{Chau Heng\} and Huang, \{Seng Wong\} and Lu, \{Chien Chen\} and Chen, \{Jiann Jong\} and Tsai, \{Shiow Chwen\} and Wang, \{Paulus S.\}",

year = "1999",

month = jun,

day = "15",

doi = "10.1002/(SICI)1097-4644(19990615)73:4<554::AID-JCB13>3.0.CO;2-L",

language = "英语",

volume = "73",

pages = "554--562",

journal = "Journal of Cellular Biochemistry",

issn = "0730-2312",

publisher = "Wiley-Liss Inc.",

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T1 - Regulation of thyroid hormones on the production of testosterone in rats

AU - Chiao, Yu Chung

AU - Lee, Hsien Yang

AU - Wang, Shyi Wu

AU - Hwang, Jiuan Jiuan

AU - Chien, Chau Heng

AU - Huang, Seng Wong

AU - Lu, Chien Chen

AU - Chen, Jiann Jong

AU - Tsai, Shiow Chwen

AU - Wang, Paulus S.

PY - 1999/6/15

Y1 - 1999/6/15

N2 - The effects of a thyroidectomy and thyroxine (T4) replacement on the spontaneous and human chorionic gonadotropin (hCG)-stimulated secretion of testosterone and the production of adenosine 3',5'-cyclic monophosphate (cAMP) in rat testes were studied. Thyroidectomy decreased the basal levels of plasma luteinizing hormone (LH) and testosterone, which delayed the maximal response of testosterone to gonadotropin-releasing hormone (GnRH) and hCG in male rats. T4 replacement in thyroparathyroidectomized (Tx) rats restored the concentrations of plasma LH and testosterone to euthyroid levels. Thyroidectomy decreased the basal release of hypothalamic GnRH, pituitary LH, and testicular testosterone as well as the LH response to GnRH and testosterone response to hCG in vitro. T4 replacement in Tx rats restored the in vitro release of GnRH, GnRH-stimulated LH release as well as hCG-stimulated testosterone release. Administration of T4 in vitro restored the release of testosterone by rat testicular interstitial cells (TICs). The increase of testosterone release in response to forskolin and androstenedione was less in TICs from Tx rats than in that from sham Tx rats. Administration of nifedipine in vitro resulted in a decrease of testosterone release by TICs from sham Tx but not from Tx rats. The basal level of cAMP in TICs was decreased by thyroidectomy. The increased accumulation of cAMP in TICs following administration of forskolin was eliminated in Tx rats. T4 replacement in Tx restored the testosterone response to forskolin. But the testosterone response to androstenedione and the cAMP response to forskolin in TICs was not restored by T4 in Tx rats. These results suggest that the inhibitory effect of a thyroidectomy on the production of testosterone in rat TICs is in part due to: 1) the decreased basal secretion of pituitary LH and its response to GnRH; 2) the decreased response of TICs to gonadotropin; and 3) the diminished production of cAMP, influx of calcium, and activity of 17β-HSD. T4 may enhance testosterone production by acting directly at the testicular interstitial cells of Tx rats.

AB - The effects of a thyroidectomy and thyroxine (T4) replacement on the spontaneous and human chorionic gonadotropin (hCG)-stimulated secretion of testosterone and the production of adenosine 3',5'-cyclic monophosphate (cAMP) in rat testes were studied. Thyroidectomy decreased the basal levels of plasma luteinizing hormone (LH) and testosterone, which delayed the maximal response of testosterone to gonadotropin-releasing hormone (GnRH) and hCG in male rats. T4 replacement in thyroparathyroidectomized (Tx) rats restored the concentrations of plasma LH and testosterone to euthyroid levels. Thyroidectomy decreased the basal release of hypothalamic GnRH, pituitary LH, and testicular testosterone as well as the LH response to GnRH and testosterone response to hCG in vitro. T4 replacement in Tx rats restored the in vitro release of GnRH, GnRH-stimulated LH release as well as hCG-stimulated testosterone release. Administration of T4 in vitro restored the release of testosterone by rat testicular interstitial cells (TICs). The increase of testosterone release in response to forskolin and androstenedione was less in TICs from Tx rats than in that from sham Tx rats. Administration of nifedipine in vitro resulted in a decrease of testosterone release by TICs from sham Tx but not from Tx rats. The basal level of cAMP in TICs was decreased by thyroidectomy. The increased accumulation of cAMP in TICs following administration of forskolin was eliminated in Tx rats. T4 replacement in Tx restored the testosterone response to forskolin. But the testosterone response to androstenedione and the cAMP response to forskolin in TICs was not restored by T4 in Tx rats. These results suggest that the inhibitory effect of a thyroidectomy on the production of testosterone in rat TICs is in part due to: 1) the decreased basal secretion of pituitary LH and its response to GnRH; 2) the decreased response of TICs to gonadotropin; and 3) the diminished production of cAMP, influx of calcium, and activity of 17β-HSD. T4 may enhance testosterone production by acting directly at the testicular interstitial cells of Tx rats.

KW - GnRH

KW - LH

KW - T

KW - Tx

KW - cAMP

UR - https://www.scopus.com/pages/publications/0033564226

U2 - 10.1002/(SICI)1097-4644(19990615)73:4<554::AID-JCB13>3.0.CO;2-L

DO - 10.1002/(SICI)1097-4644(19990615)73:4<554::AID-JCB13>3.0.CO;2-L

M3 - 文章

C2 - 10733348

AN - SCOPUS:0033564226

SN - 0730-2312

VL - 73

SP - 554

EP - 562

JO - Journal of Cellular Biochemistry

JF - Journal of Cellular Biochemistry

IS - 4

ER -

Regulation of thyroid hormones on the production of testosterone in rats

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