Relative intensities of recognition factors at two combining sites of Ralstonia solanacearum lectin (RSL) for accommodating lFucα1→, dManα1→ and Galβ1→3/4GlcNAc glycotopes

Albert M. Wu*, Jia Hua Liu, Anthony Herp, Dvora Sudakevitz, Nechama Gilboa-Garber

*Corresponding author for this work

Research output: Contribution to journalJournal Article peer-review

3 Scopus citations

Abstract

Owing to the weak reactivities of monomeric dManα1 and Galβ1→3/4GlcNAcβ (Iβ/IIβ) glycotopes with Ralstonia solanacearum lectin (RSL), their recognition roles were previously ignored. In this study, the interaction intensities of RSL toward four monomeric glycotopes lFucα1→, dManα1→ and Iβ/IIβ within two combining sites were established by both enzyme-linked lectinosorbent and inhibition assays. It was found that high density of lFucα1→ complex enhanced the recognition intensities at lFucα1→ site, polyvalent dManα1→ was essential for binding at the dManα1→ site and polyvalent I β/IIβ was required at lFucα1→ site. The peculiar recognition systems of RSL are very different from other well known microbial lectins.

Original languageEnglish
Pages (from-to)1294-1299
Number of pages6
JournalFEBS Letters
Volume586
Issue number9
DOIs
StatePublished - 07 05 2012

Keywords

  • ABH active O-glycans
  • Carbohydrate recognition domain
  • Mannan
  • Ralstonia solanacearum lectin

Fingerprint

Dive into the research topics of 'Relative intensities of recognition factors at two combining sites of Ralstonia solanacearum lectin (RSL) for accommodating lFucα1→, dManα1→ and Galβ1→3/4GlcNAc glycotopes'. Together they form a unique fingerprint.

Cite this