Resistance of cloned cytotoxic T lymphocytes to cell-mediated cytotoxicity

Ann Blakely, Keven Gorman, Hanne Ostergaard, Kathy Svoboda, Chau Ching Liu, John Ding E. Young, William R. Clark

Research output: Contribution to journalJournal Article peer-review

81 Scopus citations

Abstract

Cloned CTLs show an unusually high resistance to lysis by effector CTLs. Several cloned CTL lines in our laboratories are absolutely refractory to lysis by other cloned CTLs, either (a) directly, (b) in the presence of lectin, or (c) by PMA-induced CTLs. They can be lysed to some extent by primary CTL, although they are < 5% as sensitive as target cells normally used to assay primary CTL lytic activity. Lysis of cloned CTLs by primary CTL effector cells is not enhanced by the presence of lectin, and cloned T cells are also highly resistant to lysis by primary lymphokine-activated killer cells. Cloned CTLs are highly resistant to lysis by isolated CTL granules that contain the membranolytic poreforming protein (PFP or perforin), while non-CTL targets are highly susceptible to granule-mediated killing, indicating that cloned CTLs resist lysis not only at the intact effector level but also when soluble effector proteins are used. This resistance mechanism may explain how CTLs kill but spare themselves from being killed during the cytolytic event.
Original languageEnglish
Pages (from-to)1070-1083
Number of pages14
JournalJournal of Experimental Medicine
Volume166
Issue number4
DOIs
StatePublished - 01 10 1987
Externally publishedYes

Keywords

  • Animal
  • Antibody-Dependent Cell Cytotoxicity
  • Cell Survival
  • Clone Cells
  • Ethers
  • H-2 Antigens
  • Ionomycin
  • Mice
  • Mice, Inbred C57BL
  • Mice, Inbred CBA
  • Support, Non-U.S. Gov't
  • Support, U.S. Gov't, P.H.S.
  • T-Lymphocytes, Cytotoxic
  • Tetradecanoylphorbol Acetate

Fingerprint

Dive into the research topics of 'Resistance of cloned cytotoxic T lymphocytes to cell-mediated cytotoxicity'. Together they form a unique fingerprint.

Cite this