Reversal by pertussis toxin and N-ethylmaleimide of the facilitation of baroreceptor reflex response by somatostatin in the rat

Julie Y.H. Chan; Shyun Shyun Lin; Samuel H.H. Chan

doi:10.1016/0304-3940(92)90532-C

Reversal by pertussis toxin and N-ethylmaleimide of the facilitation of baroreceptor reflex response by somatostatin in the rat

Julie Y.H. Chan
, Shyun Shyun Lin
, Samuel H.H. Chan^*

^*Corresponding author for this work

Veterans General Hospital-Taipei
National Yang Ming Chiao Tung University

Research output: Contribution to journal › Journal Article › peer-review

2 Scopus citations

Abstract

We evaluated the transmembrane signaling mechanism that may underlie the facilitatory action of somatostatin (SOM) on baroreceptor reflex (BRR), using adult, male, Sprague-Dawley rats anesthetized with pentobarbital sodium (40 mg/kg, i.p.). Intracerebroventricular (i.c.v.) application of SOM (2 nmol) promoted a significant elevation in BRR response, induced by phenylephrine (5 μg/kg, i.v.). This potentiatory action of the tetradecapeptide was significantly reversed after pretreating animals with bilateral microinjection of pertussis toxin (25 ng) or N-ethylmaleimide (2 nmol) into the nucleus tractus solitarius (NTS), the terminal site for baroreceptor afferents. These results suggest that a pertussis toxin-sensitive GTP-binding regulatory protein, possibly G_i, may be involved in the modulation of the BRR by SOM at the NTS.

Original language	English
Pages (from-to)	267-270
Number of pages	4
Journal	Neuroscience Letters
Volume	134
Issue number	2
DOIs	https://doi.org/10.1016/0304-3940(92)90532-C
State	Published - 06 01 1992
Externally published	Yes

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This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

Baroreceptor reflex
Inhibitory GTP-binding regulatory protein
N-Ethylmaleimide
Nucleus tractus solitarius
Pertussis toxin
Rat
Somatostatin

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10.1016/0304-3940(92)90532-C

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title = "Reversal by pertussis toxin and N-ethylmaleimide of the facilitation of baroreceptor reflex response by somatostatin in the rat",

abstract = "We evaluated the transmembrane signaling mechanism that may underlie the facilitatory action of somatostatin (SOM) on baroreceptor reflex (BRR), using adult, male, Sprague-Dawley rats anesthetized with pentobarbital sodium (40 mg/kg, i.p.). Intracerebroventricular (i.c.v.) application of SOM (2 nmol) promoted a significant elevation in BRR response, induced by phenylephrine (5 μg/kg, i.v.). This potentiatory action of the tetradecapeptide was significantly reversed after pretreating animals with bilateral microinjection of pertussis toxin (25 ng) or N-ethylmaleimide (2 nmol) into the nucleus tractus solitarius (NTS), the terminal site for baroreceptor afferents. These results suggest that a pertussis toxin-sensitive GTP-binding regulatory protein, possibly Gi, may be involved in the modulation of the BRR by SOM at the NTS.",

keywords = "Baroreceptor reflex, Inhibitory GTP-binding regulatory protein, N-Ethylmaleimide, Nucleus tractus solitarius, Pertussis toxin, Rat, Somatostatin",

author = "Chan, \{Julie Y.H.\} and Lin, \{Shyun Shyun\} and Chan, \{Samuel H.H.\}",

year = "1992",

month = jan,

day = "6",

doi = "10.1016/0304-3940(92)90532-C",

language = "英语",

volume = "134",

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T1 - Reversal by pertussis toxin and N-ethylmaleimide of the facilitation of baroreceptor reflex response by somatostatin in the rat

AU - Chan, Julie Y.H.

AU - Lin, Shyun Shyun

AU - Chan, Samuel H.H.

PY - 1992/1/6

Y1 - 1992/1/6

N2 - We evaluated the transmembrane signaling mechanism that may underlie the facilitatory action of somatostatin (SOM) on baroreceptor reflex (BRR), using adult, male, Sprague-Dawley rats anesthetized with pentobarbital sodium (40 mg/kg, i.p.). Intracerebroventricular (i.c.v.) application of SOM (2 nmol) promoted a significant elevation in BRR response, induced by phenylephrine (5 μg/kg, i.v.). This potentiatory action of the tetradecapeptide was significantly reversed after pretreating animals with bilateral microinjection of pertussis toxin (25 ng) or N-ethylmaleimide (2 nmol) into the nucleus tractus solitarius (NTS), the terminal site for baroreceptor afferents. These results suggest that a pertussis toxin-sensitive GTP-binding regulatory protein, possibly Gi, may be involved in the modulation of the BRR by SOM at the NTS.

AB - We evaluated the transmembrane signaling mechanism that may underlie the facilitatory action of somatostatin (SOM) on baroreceptor reflex (BRR), using adult, male, Sprague-Dawley rats anesthetized with pentobarbital sodium (40 mg/kg, i.p.). Intracerebroventricular (i.c.v.) application of SOM (2 nmol) promoted a significant elevation in BRR response, induced by phenylephrine (5 μg/kg, i.v.). This potentiatory action of the tetradecapeptide was significantly reversed after pretreating animals with bilateral microinjection of pertussis toxin (25 ng) or N-ethylmaleimide (2 nmol) into the nucleus tractus solitarius (NTS), the terminal site for baroreceptor afferents. These results suggest that a pertussis toxin-sensitive GTP-binding regulatory protein, possibly Gi, may be involved in the modulation of the BRR by SOM at the NTS.

KW - Baroreceptor reflex

KW - Inhibitory GTP-binding regulatory protein

KW - N-Ethylmaleimide

KW - Nucleus tractus solitarius

KW - Pertussis toxin

KW - Rat

KW - Somatostatin

UR - https://www.scopus.com/pages/publications/0026515935

U2 - 10.1016/0304-3940(92)90532-C

DO - 10.1016/0304-3940(92)90532-C

M3 - 文章

C2 - 1350336

AN - SCOPUS:0026515935

SN - 0304-3940

VL - 134

SP - 267

EP - 270

JO - Neuroscience Letters

JF - Neuroscience Letters

IS - 2

ER -

Reversal by pertussis toxin and N-ethylmaleimide of the facilitation of baroreceptor reflex response by somatostatin in the rat

Abstract

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Keywords

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