Reverse transcriptase-polymerase chain reaction for amantadine susceptibility testing of influenza viruses

P. C. Suen; K. C. Tsao; T. L. Wu; H. C. Ning; T. Z. Liu; S. R. Shin

doi:10.1023/A:1018454031252

Reverse transcriptase-polymerase chain reaction for amantadine susceptibility testing of influenza viruses

P. C. Suen^*, K. C. Tsao, T. L. Wu, H. C. Ning, T. Z. Liu, S. R. Shin

^*Corresponding author for this work

Research output: Contribution to journal › Journal Article › peer-review

Abstract

The clinical use of amantadine for influenza A viruses infection is increasing. A novel quantitative reverse transcriptase-polymerase chain reaction (RT-PCR) assay was developed to determine the IC₅₀ (dose of 50% inhibitory) of amantadine. The traditional amantadine susceptibility testing by plaque inhibition assay took 3 days to observe plaque formation. In contrast, the proposed assay took 3 hours to detect vital RNA. The RT-PCR products can be directly sequenced for screening and identifying the resistant strains.

Original language	English
Pages (from-to)	873-878
Number of pages	6
Journal	Biotechnology Techniques
Volume	11
Issue number	12
DOIs	https://doi.org/10.1023/A:1018454031252
State	Published - 1997
Externally published	Yes

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title = "Reverse transcriptase-polymerase chain reaction for amantadine susceptibility testing of influenza viruses",

abstract = "The clinical use of amantadine for influenza A viruses infection is increasing. A novel quantitative reverse transcriptase-polymerase chain reaction (RT-PCR) assay was developed to determine the IC50 (dose of 50% inhibitory) of amantadine. The traditional amantadine susceptibility testing by plaque inhibition assay took 3 days to observe plaque formation. In contrast, the proposed assay took 3 hours to detect vital RNA. The RT-PCR products can be directly sequenced for screening and identifying the resistant strains.",

author = "Suen, {P. C.} and Tsao, {K. C.} and Wu, {T. L.} and Ning, {H. C.} and Liu, {T. Z.} and Shin, {S. R.}",

year = "1997",

doi = "10.1023/A:1018454031252",

language = "英语",

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pages = "873--878",

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T1 - Reverse transcriptase-polymerase chain reaction for amantadine susceptibility testing of influenza viruses

AU - Suen, P. C.

AU - Tsao, K. C.

AU - Wu, T. L.

AU - Ning, H. C.

AU - Liu, T. Z.

AU - Shin, S. R.

PY - 1997

Y1 - 1997

N2 - The clinical use of amantadine for influenza A viruses infection is increasing. A novel quantitative reverse transcriptase-polymerase chain reaction (RT-PCR) assay was developed to determine the IC50 (dose of 50% inhibitory) of amantadine. The traditional amantadine susceptibility testing by plaque inhibition assay took 3 days to observe plaque formation. In contrast, the proposed assay took 3 hours to detect vital RNA. The RT-PCR products can be directly sequenced for screening and identifying the resistant strains.

AB - The clinical use of amantadine for influenza A viruses infection is increasing. A novel quantitative reverse transcriptase-polymerase chain reaction (RT-PCR) assay was developed to determine the IC50 (dose of 50% inhibitory) of amantadine. The traditional amantadine susceptibility testing by plaque inhibition assay took 3 days to observe plaque formation. In contrast, the proposed assay took 3 hours to detect vital RNA. The RT-PCR products can be directly sequenced for screening and identifying the resistant strains.

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U2 - 10.1023/A:1018454031252

DO - 10.1023/A:1018454031252

M3 - 文章

AN - SCOPUS:0031445042

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VL - 11

SP - 873

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JO - Biotechnology Techniques

JF - Biotechnology Techniques

IS - 12

ER -

Reverse transcriptase-polymerase chain reaction for amantadine susceptibility testing of influenza viruses

Abstract

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