Reverse transcriptase-polymerase chain reaction for amantadine susceptibility testing of influenza viruses

P. C. Suen; K. C. Tsao; T. L. Wu; H. C. Ning; T. Z. Liu; S. R. Shin

doi:10.1023/A:1018454031252

Reverse transcriptase-polymerase chain reaction for amantadine susceptibility testing of influenza viruses

P. C. Suen^*
, K. C. Tsao
, T. L. Wu
, H. C. Ning
, T. Z. Liu
, S. R. Shin

^*Corresponding author for this work

Chang Gung Memorial Hospital
Chang Gung University
Yuan's General Hospital

Research output: Contribution to journal › Journal Article › peer-review

Abstract

The clinical use of amantadine for influenza A viruses infection is increasing. A novel quantitative reverse transcriptase-polymerase chain reaction (RT-PCR) assay was developed to determine the IC₅₀ (dose of 50% inhibitory) of amantadine. The traditional amantadine susceptibility testing by plaque inhibition assay took 3 days to observe plaque formation. In contrast, the proposed assay took 3 hours to detect vital RNA. The RT-PCR products can be directly sequenced for screening and identifying the resistant strains.

Original language	English
Pages (from-to)	873-878
Number of pages	6
Journal	Biotechnology Techniques
Volume	11
Issue number	12
DOIs	https://doi.org/10.1023/A:1018454031252
State	Published - 1997
Externally published	Yes

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

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10.1023/A:1018454031252

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title = "Reverse transcriptase-polymerase chain reaction for amantadine susceptibility testing of influenza viruses",

abstract = "The clinical use of amantadine for influenza A viruses infection is increasing. A novel quantitative reverse transcriptase-polymerase chain reaction (RT-PCR) assay was developed to determine the IC50 (dose of 50\% inhibitory) of amantadine. The traditional amantadine susceptibility testing by plaque inhibition assay took 3 days to observe plaque formation. In contrast, the proposed assay took 3 hours to detect vital RNA. The RT-PCR products can be directly sequenced for screening and identifying the resistant strains.",

author = "Suen, \{P. C.\} and Tsao, \{K. C.\} and Wu, \{T. L.\} and Ning, \{H. C.\} and Liu, \{T. Z.\} and Shin, \{S. R.\}",

year = "1997",

doi = "10.1023/A:1018454031252",

language = "英语",

volume = "11",

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T1 - Reverse transcriptase-polymerase chain reaction for amantadine susceptibility testing of influenza viruses

AU - Suen, P. C.

AU - Tsao, K. C.

AU - Wu, T. L.

AU - Ning, H. C.

AU - Liu, T. Z.

AU - Shin, S. R.

PY - 1997

Y1 - 1997

N2 - The clinical use of amantadine for influenza A viruses infection is increasing. A novel quantitative reverse transcriptase-polymerase chain reaction (RT-PCR) assay was developed to determine the IC50 (dose of 50% inhibitory) of amantadine. The traditional amantadine susceptibility testing by plaque inhibition assay took 3 days to observe plaque formation. In contrast, the proposed assay took 3 hours to detect vital RNA. The RT-PCR products can be directly sequenced for screening and identifying the resistant strains.

AB - The clinical use of amantadine for influenza A viruses infection is increasing. A novel quantitative reverse transcriptase-polymerase chain reaction (RT-PCR) assay was developed to determine the IC50 (dose of 50% inhibitory) of amantadine. The traditional amantadine susceptibility testing by plaque inhibition assay took 3 days to observe plaque formation. In contrast, the proposed assay took 3 hours to detect vital RNA. The RT-PCR products can be directly sequenced for screening and identifying the resistant strains.

UR - https://www.scopus.com/pages/publications/0031445042

U2 - 10.1023/A:1018454031252

DO - 10.1023/A:1018454031252

M3 - 文章

AN - SCOPUS:0031445042

SN - 0951-208X

VL - 11

SP - 873

EP - 878

JO - Biotechnology Techniques

JF - Biotechnology Techniques

IS - 12

ER -

Reverse transcriptase-polymerase chain reaction for amantadine susceptibility testing of influenza viruses

Abstract

UN SDGs

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