Role of DNA methyltransferase 1 in pharyngeal cancer related to treatment resistance

Chih Cheng Chen; Wen Cheng Chen; Wen Hung Wang; Chang Hsien Lu; Paul Yang Lin; Kuan Der Lee; Miao Fen Chen

doi:10.1002/hed.21586

Role of DNA methyltransferase 1 in pharyngeal cancer related to treatment resistance

Chih Cheng Chen, Wen Cheng Chen, Wen Hung Wang, Chang Hsien Lu, Paul Yang Lin, Kuan Der Lee, Miao Fen Chen^*

^*Corresponding author for this work

Research output: Contribution to journal › Journal Article › peer-review

11 Scopus citations

Abstract

Background: The purpose of the present study was to highlight the role of DNA methyltransferase 1 (DNMT1) and its potential for improving treatment and informing prognosis for pharyngeal cancer. Methods: Human pharyngeal cancer cell lines FaDu and its derivative FaDu-C225-R were selected for cellular experiments. Furthermore, 95 pharyngeal cancer tissue specimens were analyzed by immunohistochemical staining. Results: DNMT1 was over-expressed in pharyngeal cancer specimens and cells with activated interleukin (IL)-6 signaling. When DNMT1 activity was blocked, accelerated tumor growth and treatment resistance were overcome as demonstrated by cell culture and animal experiments. The reduction in DNMT1 was associated with increased apoptosis, cell cycle arrest, and augmented irradiation-induced free radical levels and DNA damage. Furthermore, positive staining for DNMT1 in clinical cancer specimens was significantly linked to lower rates of response to treatments and shorter survival of patients with pharyngeal cancer. Conclusion: DNMT1 may be a significant clinical predictor and a potential treatment strategy against head and neck cancer.

Original language	English
Pages (from-to)	1132-1143
Number of pages	12
Journal	Head and Neck
Volume	33
Issue number	8
DOIs	https://doi.org/10.1002/hed.21586
State	Published - 08 2011

Keywords

DNMT1
IL-6
pharyngeal cancer

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1002/hed.21586

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@article{3b104ebdd3a741ac8ed26e9bd0798e85,

title = "Role of DNA methyltransferase 1 in pharyngeal cancer related to treatment resistance",

abstract = "Background: The purpose of the present study was to highlight the role of DNA methyltransferase 1 (DNMT1) and its potential for improving treatment and informing prognosis for pharyngeal cancer. Methods: Human pharyngeal cancer cell lines FaDu and its derivative FaDu-C225-R were selected for cellular experiments. Furthermore, 95 pharyngeal cancer tissue specimens were analyzed by immunohistochemical staining. Results: DNMT1 was over-expressed in pharyngeal cancer specimens and cells with activated interleukin (IL)-6 signaling. When DNMT1 activity was blocked, accelerated tumor growth and treatment resistance were overcome as demonstrated by cell culture and animal experiments. The reduction in DNMT1 was associated with increased apoptosis, cell cycle arrest, and augmented irradiation-induced free radical levels and DNA damage. Furthermore, positive staining for DNMT1 in clinical cancer specimens was significantly linked to lower rates of response to treatments and shorter survival of patients with pharyngeal cancer. Conclusion: DNMT1 may be a significant clinical predictor and a potential treatment strategy against head and neck cancer.",

keywords = "DNMT1, IL-6, pharyngeal cancer",

author = "Chen, {Chih Cheng} and Chen, {Wen Cheng} and Wang, {Wen Hung} and Lu, {Chang Hsien} and Lin, {Paul Yang} and Lee, {Kuan Der} and Chen, {Miao Fen}",

year = "2011",

month = aug,

doi = "10.1002/hed.21586",

language = "英语",

volume = "33",

pages = "1132--1143",

journal = "Head and Neck",

issn = "1043-3074",

publisher = "John Wiley & Sons Inc.",

number = "8",

}

TY - JOUR

T1 - Role of DNA methyltransferase 1 in pharyngeal cancer related to treatment resistance

AU - Chen, Chih Cheng

AU - Chen, Wen Cheng

AU - Wang, Wen Hung

AU - Lu, Chang Hsien

AU - Lin, Paul Yang

AU - Lee, Kuan Der

AU - Chen, Miao Fen

PY - 2011/8

Y1 - 2011/8

N2 - Background: The purpose of the present study was to highlight the role of DNA methyltransferase 1 (DNMT1) and its potential for improving treatment and informing prognosis for pharyngeal cancer. Methods: Human pharyngeal cancer cell lines FaDu and its derivative FaDu-C225-R were selected for cellular experiments. Furthermore, 95 pharyngeal cancer tissue specimens were analyzed by immunohistochemical staining. Results: DNMT1 was over-expressed in pharyngeal cancer specimens and cells with activated interleukin (IL)-6 signaling. When DNMT1 activity was blocked, accelerated tumor growth and treatment resistance were overcome as demonstrated by cell culture and animal experiments. The reduction in DNMT1 was associated with increased apoptosis, cell cycle arrest, and augmented irradiation-induced free radical levels and DNA damage. Furthermore, positive staining for DNMT1 in clinical cancer specimens was significantly linked to lower rates of response to treatments and shorter survival of patients with pharyngeal cancer. Conclusion: DNMT1 may be a significant clinical predictor and a potential treatment strategy against head and neck cancer.

AB - Background: The purpose of the present study was to highlight the role of DNA methyltransferase 1 (DNMT1) and its potential for improving treatment and informing prognosis for pharyngeal cancer. Methods: Human pharyngeal cancer cell lines FaDu and its derivative FaDu-C225-R were selected for cellular experiments. Furthermore, 95 pharyngeal cancer tissue specimens were analyzed by immunohistochemical staining. Results: DNMT1 was over-expressed in pharyngeal cancer specimens and cells with activated interleukin (IL)-6 signaling. When DNMT1 activity was blocked, accelerated tumor growth and treatment resistance were overcome as demonstrated by cell culture and animal experiments. The reduction in DNMT1 was associated with increased apoptosis, cell cycle arrest, and augmented irradiation-induced free radical levels and DNA damage. Furthermore, positive staining for DNMT1 in clinical cancer specimens was significantly linked to lower rates of response to treatments and shorter survival of patients with pharyngeal cancer. Conclusion: DNMT1 may be a significant clinical predictor and a potential treatment strategy against head and neck cancer.

KW - DNMT1

KW - IL-6

KW - pharyngeal cancer

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U2 - 10.1002/hed.21586

DO - 10.1002/hed.21586

M3 - 文章

C2 - 21284050

AN - SCOPUS:79960401628

SN - 1043-3074

VL - 33

SP - 1132

EP - 1143

JO - Head and Neck

JF - Head and Neck

IS - 8

ER -

Role of DNA methyltransferase 1 in pharyngeal cancer related to treatment resistance

Abstract

Keywords

UN SDGs

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