Screening of aptamers specific to colorectal cancer cells and stem cells by utilizing On-chip Cell-SELEX

Lien Yu Hung; Chih Hung Wang; Yu Jui Che; Chien Yu Fu; Hwan You Chang; Kuan Wang; Gwo Bin Lee

doi:10.1038/srep10326

Screening of aptamers specific to colorectal cancer cells and stem cells by utilizing On-chip Cell-SELEX

Lien Yu Hung, Chih Hung Wang, Yu Jui Che, Chien Yu Fu, Hwan You Chang, Kuan Wang, Gwo Bin Lee^*

^*Corresponding author for this work

Research output: Contribution to journal › Journal Article › peer-review

58 Scopus citations

Abstract

Colorectal cancer (CRC) is the most frequently diagnosed cancer around the world, causing about 700,000 deaths every year. It is clear now that a small fraction of CRC, named colorectal cancer stem cells (CSCs) exhibiting self-renewal and extensive proliferative activities, are hard to be eradicated. Unfortunately, highly specific biomarkers for colorectal CSC (CR-CSCs) are lacking that prohibits the development of effective therapeutic strategies. This study designed and manufactured a novel microfluidic system capable of performing a fully automated cell-based, systematic evolution of ligands by exponential enrichment (SELEX) process. Eight CR-CSC/CRC-specific aptamers were successfully selected using the microfluidic chip. Three of the aptamers showed high affinities towards their respective target cells with a dissociation constant of 27.4, 28.5 and 12.3 nM, which are comparable to that of antibodies.

Original language	English
Article number	10326
Journal	Scientific Reports
Volume	5
DOIs	https://doi.org/10.1038/srep10326
State	Published - 22 05 2015
Externally published	Yes

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title = "Screening of aptamers specific to colorectal cancer cells and stem cells by utilizing On-chip Cell-SELEX",

abstract = "Colorectal cancer (CRC) is the most frequently diagnosed cancer around the world, causing about 700,000 deaths every year. It is clear now that a small fraction of CRC, named colorectal cancer stem cells (CSCs) exhibiting self-renewal and extensive proliferative activities, are hard to be eradicated. Unfortunately, highly specific biomarkers for colorectal CSC (CR-CSCs) are lacking that prohibits the development of effective therapeutic strategies. This study designed and manufactured a novel microfluidic system capable of performing a fully automated cell-based, systematic evolution of ligands by exponential enrichment (SELEX) process. Eight CR-CSC/CRC-specific aptamers were successfully selected using the microfluidic chip. Three of the aptamers showed high affinities towards their respective target cells with a dissociation constant of 27.4, 28.5 and 12.3 nM, which are comparable to that of antibodies.",

author = "Hung, {Lien Yu} and Wang, {Chih Hung} and Che, {Yu Jui} and Fu, {Chien Yu} and Chang, {Hwan You} and Kuan Wang and Lee, {Gwo Bin}",

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T1 - Screening of aptamers specific to colorectal cancer cells and stem cells by utilizing On-chip Cell-SELEX

AU - Hung, Lien Yu

AU - Wang, Chih Hung

AU - Che, Yu Jui

AU - Fu, Chien Yu

AU - Chang, Hwan You

AU - Wang, Kuan

AU - Lee, Gwo Bin

PY - 2015/5/22

Y1 - 2015/5/22

N2 - Colorectal cancer (CRC) is the most frequently diagnosed cancer around the world, causing about 700,000 deaths every year. It is clear now that a small fraction of CRC, named colorectal cancer stem cells (CSCs) exhibiting self-renewal and extensive proliferative activities, are hard to be eradicated. Unfortunately, highly specific biomarkers for colorectal CSC (CR-CSCs) are lacking that prohibits the development of effective therapeutic strategies. This study designed and manufactured a novel microfluidic system capable of performing a fully automated cell-based, systematic evolution of ligands by exponential enrichment (SELEX) process. Eight CR-CSC/CRC-specific aptamers were successfully selected using the microfluidic chip. Three of the aptamers showed high affinities towards their respective target cells with a dissociation constant of 27.4, 28.5 and 12.3 nM, which are comparable to that of antibodies.

AB - Colorectal cancer (CRC) is the most frequently diagnosed cancer around the world, causing about 700,000 deaths every year. It is clear now that a small fraction of CRC, named colorectal cancer stem cells (CSCs) exhibiting self-renewal and extensive proliferative activities, are hard to be eradicated. Unfortunately, highly specific biomarkers for colorectal CSC (CR-CSCs) are lacking that prohibits the development of effective therapeutic strategies. This study designed and manufactured a novel microfluidic system capable of performing a fully automated cell-based, systematic evolution of ligands by exponential enrichment (SELEX) process. Eight CR-CSC/CRC-specific aptamers were successfully selected using the microfluidic chip. Three of the aptamers showed high affinities towards their respective target cells with a dissociation constant of 27.4, 28.5 and 12.3 nM, which are comparable to that of antibodies.

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Screening of aptamers specific to colorectal cancer cells and stem cells by utilizing On-chip Cell-SELEX

Abstract

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