TY - JOUR
T1 - Secondary metabolites and bioactivities of aspergillus ochraceopetaliformis isolated from anthurium brownii
AU - Hu, Hao Chun
AU - Li, Chi Ying
AU - Tsai, Yi Hong
AU - Yang, Dai Yun
AU - Wu, Yang Chang
AU - Hwang, Tsong Long
AU - Chen, Shu Li
AU - Fülöp, Ferenc
AU - Hunyadi, Attila
AU - Yen, Chia Hung
AU - Cheng, Yuan Bin
AU - Chang, Fang Rong
N1 - Publisher Copyright:
Copyright © 2020 American Chemical Society.
PY - 2020/8/25
Y1 - 2020/8/25
N2 - Five new polyketides, asperochrapyran (1) and asperochralactones A-D (2-5), along with 12 known polyketides (6-17), were obtained from the fungal strain Aspergillus ochraceopetaliformis. Structures of all isolates were elucidated by their spectroscopic parameters. The relative configurations of the new compounds were deduced by the data of coupling constants and NOESY spectra. The absolute configurations were determined by the comparison of experimental and calculated ECD spectra. Moreover, the plausible biosynthesis pathway of major isolates was proposed as well. Anti-inflammatory activity of compounds 5 and 7-17 were evaluated with human neutrophils in response to the stimulation of formyl-methionyl-leucyl phenylalanine (fMLP). Asperlactone (9), aspyrone (13), and (-)-(3R)-mellein (14) exerted superoxide anion inhibition at 30 ± 9%, 29 ± 9%, and 26 ± 12%, respectively, at 10 μM. The capacities of asperlactone (9), aspilactonol B (10), penicillic acid (12), and (-)-(3R)-mellein (14) in elastase release inhibition were revealed as 25 ± 4%, 38 ± 8%, 25 ± 5%, and 34 ± 9%, respectively, at 10 μM.
AB - Five new polyketides, asperochrapyran (1) and asperochralactones A-D (2-5), along with 12 known polyketides (6-17), were obtained from the fungal strain Aspergillus ochraceopetaliformis. Structures of all isolates were elucidated by their spectroscopic parameters. The relative configurations of the new compounds were deduced by the data of coupling constants and NOESY spectra. The absolute configurations were determined by the comparison of experimental and calculated ECD spectra. Moreover, the plausible biosynthesis pathway of major isolates was proposed as well. Anti-inflammatory activity of compounds 5 and 7-17 were evaluated with human neutrophils in response to the stimulation of formyl-methionyl-leucyl phenylalanine (fMLP). Asperlactone (9), aspyrone (13), and (-)-(3R)-mellein (14) exerted superoxide anion inhibition at 30 ± 9%, 29 ± 9%, and 26 ± 12%, respectively, at 10 μM. The capacities of asperlactone (9), aspilactonol B (10), penicillic acid (12), and (-)-(3R)-mellein (14) in elastase release inhibition were revealed as 25 ± 4%, 38 ± 8%, 25 ± 5%, and 34 ± 9%, respectively, at 10 μM.
UR - http://www.scopus.com/inward/record.url?scp=85091047194&partnerID=8YFLogxK
U2 - 10.1021/acsomega.0c02489
DO - 10.1021/acsomega.0c02489
M3 - 文章
AN - SCOPUS:85091047194
SN - 2470-1343
VL - 5
SP - 20991
EP - 20999
JO - ACS Omega
JF - ACS Omega
IS - 33
ER -