Abstract
Diagnosis and treatment of periprosthetic joint infection (PJI) have been reported as one of the most costly complications after joint arthroplasty. This work demonstrates point-of-care applications for screening aptamers specific to α-defensin human neutrophil peptide (HNP)-1 used as a synovial fluid biomarker for detecting PJI. An integrated microfluidic system capable of performing protein-based systematic evolution of ligands by exponential enrichment (protein-SELEX) process on-chip was developed. Aptamers detecting HNP-1 were screened successfully and may be applied in liquid biopsy in the near future.
| Original language | English |
|---|---|
| Title of host publication | MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences |
| Publisher | Chemical and Biological Microsystems Society |
| Pages | 177-178 |
| Number of pages | 2 |
| ISBN (Electronic) | 9781733419031 |
| State | Published - 2021 |
| Event | 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2021 - Palm Springs, Virtual, United States Duration: 10 10 2021 → 14 10 2021 |
Publication series
| Name | MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences |
|---|
Conference
| Conference | 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2021 |
|---|---|
| Country/Territory | United States |
| City | Palm Springs, Virtual |
| Period | 10/10/21 → 14/10/21 |
Bibliographical note
Publisher Copyright:© 2021 MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences. All rights reserved.
Keywords
- Aptamers
- HNP-1
- Microfluidics
- Periprosthetic joint infection
- SELEX