Self-renewal and differentiation of adipose-derived stem cells (ADSCs) stimulated by multi-axial tensile strain in a pneumatic microdevice

Chih Hao Chiu, Yun Wen Tong, Wen Ling Yeh, Kin Fong Lei*, Alvin Chao Yu Chen

*Corresponding author for this work

Research output: Contribution to journalJournal Article peer-review

7 Scopus citations

Abstract

Adipose-derived stem cells (ADSCs) were suggested for treating degenerative osteoarthritis, suppressing inflammatory responses, and repairing damaged soft tissues. Moreover, the ADSCs have the potential to undergo self-renewal and differentiate into bone, tendon, cartilage, and ligament. Recently, investigation of the self-renewal and differentiation of the ADSCs has become an attractive area. In this work, a pneumatic microdevice has been developed to study the gene expression of the ADSCs after the stimulation of multi-axial tensile strain. The ADSCs were cultured on the microdevice and experienced multi-axial tensile strain during a three-day culture course. Self-renewal and differentiation abilities were investigated by mRNA expressions of NANOG, sex determining region Y-box 2 (SOX2), octamer-binding transcription factor 4 (OCT4), sex determining region Y-box9 (SOX9), peroxisome proliferator-activated receptor gamma (PPAR-γ), and runt-related transcription factor 2 (RUNX2). The result showed that the genes related self-renewal were significantly up-regulated after the tensile stimulation. Higher proliferation ratio of the ADSCs was also shown by cell viability assay. The microdevice provides a promising platform for cell-based study under mechanical tensile stimulation.

Original languageEnglish
Article number607
JournalMicromachines
Volume9
Issue number11
DOIs
StatePublished - 19 11 2018

Bibliographical note

Publisher Copyright:
© 2018 by the authors.

Keywords

  • Adipose-derived stem cells
  • Differentiation
  • Microdevice
  • Self-renewal
  • Tensile stimulation

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