Sensitive detection of platelet-derived growth factor through surface-enhanced Raman scattering

A surface-enhanced Raman scattering (SERS) assay using two different nanomaterials has been demonstrated for highly sensitive and selective detection of platelet-derived growth factor (PDGF). Gold nanoparticles (Au NPs; 13 nm) are conjugated with aptamer (Apt) and 4-mercaptobenzoic acid (MBA) as the recognition element and reporter, respectively, while Au pearl necklace nanomaterials (Au PNNs) are used for generating reproducible and enhanced SERS signal of 4-MBA. The Apt/MBA-Au NPs bind PDGF through a specific interaction between Apt and PDGF in a fashion of 2:1, leading to concentration of the analyte and removal of the sample matrix. Through electrostatic interaction, the PDGF-Apt/MBA-Au NPs complexes form aggregates with Au PNNs, leading to an enhanced Raman signal of 4-MBA. Au PNNs allow enhancement factors up to 1.3 × 10⁷ and relative standard deviations of Raman signals for 4-MBA down to 15% (five measurements). The assay allows detection of PDGF BB down to 0.5 pM, with linearity of the Raman signal of 4-MBA against the concentration of PDGF over 1-50 pM. Having advantages of sensitivity and reproducibility, this assay has been further applied for the determination of the concentration of PDGF in urine samples, showing its great potential for ultrasensitive analysis of target proteins in biological samples.