TY - JOUR
T1 - Sensitive dual-target electrochemiluminescence biosensor for simultaneous miRNA detection using magnetic carbon nanotubes and resonance energy transfer
AU - Liu, Chi Hsien
AU - Malla, Pravanjan
AU - Wu, Wei Chi
AU - Ganesan, Selvaganapathy
N1 - Publisher Copyright:
© 2024 Elsevier B.V.
PY - 2025/2/1
Y1 - 2025/2/1
N2 - Electrochemiluminescence (ECL) initiated precisely by potential can effectively control the timing and increase the intensity of chemiluminescence, making it a vital light source for optical biosensors. Chemiluminescence resonance energy transfer (CRET) involves non-radiative energy transfer from a chemiluminescent donor to an appropriate acceptor molecule, eliminating the need for a laser light and offering a low background. Magnetic carbon nanotubes (MCNTs) possess magnetic responsiveness, high conductivity, and ease of modification. We designed and synthesized multifunctional magnetic carriers to capture miRNA targets (miRNA-183 or miRNA-21). In the presence of luminol, peroxidase catalyzes the ECL reaction at an oxidative potential of 250 mV, transferring 425 nm energy to excite two organic fluorescent dyes (Pacific Blue and Alexa fluor) on the detection probes. These dyes emit distinct fluorescence, allowing for the simultaneous detection of two miRNAs. This simple and controllable ECL design, combined with functionalized MCNTs and a high-efficiency CRET mechanism, enables simultaneous dual-miRNA detection within 30 min. The detection limits for miRNA-21 and miRNA-183 in human serum are 0.38 fM and 0.49 fM, respectively. The developed ECL detection system demonstrates high energy transfer efficiency, reasonable specificity, and selectivity, which provides a promising approach for rapid multi-target detection in on-site diagnostics.
AB - Electrochemiluminescence (ECL) initiated precisely by potential can effectively control the timing and increase the intensity of chemiluminescence, making it a vital light source for optical biosensors. Chemiluminescence resonance energy transfer (CRET) involves non-radiative energy transfer from a chemiluminescent donor to an appropriate acceptor molecule, eliminating the need for a laser light and offering a low background. Magnetic carbon nanotubes (MCNTs) possess magnetic responsiveness, high conductivity, and ease of modification. We designed and synthesized multifunctional magnetic carriers to capture miRNA targets (miRNA-183 or miRNA-21). In the presence of luminol, peroxidase catalyzes the ECL reaction at an oxidative potential of 250 mV, transferring 425 nm energy to excite two organic fluorescent dyes (Pacific Blue and Alexa fluor) on the detection probes. These dyes emit distinct fluorescence, allowing for the simultaneous detection of two miRNAs. This simple and controllable ECL design, combined with functionalized MCNTs and a high-efficiency CRET mechanism, enables simultaneous dual-miRNA detection within 30 min. The detection limits for miRNA-21 and miRNA-183 in human serum are 0.38 fM and 0.49 fM, respectively. The developed ECL detection system demonstrates high energy transfer efficiency, reasonable specificity, and selectivity, which provides a promising approach for rapid multi-target detection in on-site diagnostics.
KW - Dual-target biosensor
KW - Electrochemiluminescence
KW - Magnetic carbon nanotubes
KW - MicroRNA
KW - Resonance energy transfer
UR - http://www.scopus.com/inward/record.url?scp=85208111029&partnerID=8YFLogxK
U2 - 10.1016/j.snb.2024.136876
DO - 10.1016/j.snb.2024.136876
M3 - 文章
AN - SCOPUS:85208111029
SN - 0925-4005
VL - 424
JO - Sensors and Actuators B: Chemical
JF - Sensors and Actuators B: Chemical
M1 - 136876
ER -