Sequence analysis of anerwinia stewartiiplasmid, psw100

Jen Fen Fu; Han Chang Chang; Yih Ming Chen; Yu Sun Chang; Shih Tung Liu

doi:10.1006/plas.1995.9999

Sequence analysis of anerwinia stewartiiplasmid, psw100

Jen Fen Fu, Han Chang Chang, Yih Ming Chen, Yu Sun Chang, Shih Tung Liu^*

^*Corresponding author for this work

Research output: Contribution to journal › Journal Article › peer-review

21 Scopus citations

Abstract

The nucleotide sequence of the smallest plasmid of Erwinia stewartiiSW2 was determined. This plasmid, pSW100 (4272 bp), consists of a 702-bp region homologous to the origins of replication of plasmids p15A, ColE1, and ColA. Plasmid pSW100 also contains sequences homologous to the bomregion and mobCABD genes of ColE1, except that a single-base deletion in mobA has been detected. This deletion did not affect the mobilization ability of pSW100 by an endogenous conjugative plasmid of E. stewartii SW2, pDC250. Plasmid pSW100 also has a 1596-bp open reading frame with five 132-bp perfect repeats. However, this open reading frame and the repeats are not required for plasmid replication. Furthermore, curing of pSW100 did not cause any phenotypic change, suggesting that this plasmid is not essential for the survival of the organism.

Original language	English
Pages (from-to)	75-84
Number of pages	10
Journal	Plasmid
Volume	34
Issue number	2
DOIs	https://doi.org/10.1006/plas.1995.9999
State	Published - 09 1995

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title = "Sequence analysis of anerwinia stewartiiplasmid, psw100",

abstract = "The nucleotide sequence of the smallest plasmid of Erwinia stewartiiSW2 was determined. This plasmid, pSW100 (4272 bp), consists of a 702-bp region homologous to the origins of replication of plasmids p15A, ColE1, and ColA. Plasmid pSW100 also contains sequences homologous to the bomregion and mobCABD genes of ColE1, except that a single-base deletion in mobA has been detected. This deletion did not affect the mobilization ability of pSW100 by an endogenous conjugative plasmid of E. stewartii SW2, pDC250. Plasmid pSW100 also has a 1596-bp open reading frame with five 132-bp perfect repeats. However, this open reading frame and the repeats are not required for plasmid replication. Furthermore, curing of pSW100 did not cause any phenotypic change, suggesting that this plasmid is not essential for the survival of the organism.",

author = "Fu, \{Jen Fen\} and Chang, \{Han Chang\} and Chen, \{Yih Ming\} and Chang, \{Yu Sun\} and Liu, \{Shih Tung\}",

year = "1995",

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doi = "10.1006/plas.1995.9999",

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T1 - Sequence analysis of anerwinia stewartiiplasmid, psw100

AU - Fu, Jen Fen

AU - Chang, Han Chang

AU - Chen, Yih Ming

AU - Chang, Yu Sun

AU - Liu, Shih Tung

PY - 1995/9

Y1 - 1995/9

N2 - The nucleotide sequence of the smallest plasmid of Erwinia stewartiiSW2 was determined. This plasmid, pSW100 (4272 bp), consists of a 702-bp region homologous to the origins of replication of plasmids p15A, ColE1, and ColA. Plasmid pSW100 also contains sequences homologous to the bomregion and mobCABD genes of ColE1, except that a single-base deletion in mobA has been detected. This deletion did not affect the mobilization ability of pSW100 by an endogenous conjugative plasmid of E. stewartii SW2, pDC250. Plasmid pSW100 also has a 1596-bp open reading frame with five 132-bp perfect repeats. However, this open reading frame and the repeats are not required for plasmid replication. Furthermore, curing of pSW100 did not cause any phenotypic change, suggesting that this plasmid is not essential for the survival of the organism.

AB - The nucleotide sequence of the smallest plasmid of Erwinia stewartiiSW2 was determined. This plasmid, pSW100 (4272 bp), consists of a 702-bp region homologous to the origins of replication of plasmids p15A, ColE1, and ColA. Plasmid pSW100 also contains sequences homologous to the bomregion and mobCABD genes of ColE1, except that a single-base deletion in mobA has been detected. This deletion did not affect the mobilization ability of pSW100 by an endogenous conjugative plasmid of E. stewartii SW2, pDC250. Plasmid pSW100 also has a 1596-bp open reading frame with five 132-bp perfect repeats. However, this open reading frame and the repeats are not required for plasmid replication. Furthermore, curing of pSW100 did not cause any phenotypic change, suggesting that this plasmid is not essential for the survival of the organism.

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U2 - 10.1006/plas.1995.9999

DO - 10.1006/plas.1995.9999

M3 - 文章

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AN - SCOPUS:0029103273

SN - 0147-619X

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JO - Plasmid

JF - Plasmid

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ER -

Sequence analysis of anerwinia stewartiiplasmid, psw100

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