Sequencing and analysis of the large virulence plasmid pLVPK of Klebsiella pneumoniae CG43

Ying Tsong Chen, Hwan You Chang, Yi Chyi Lai, Chao Chi Pan, Shih Feng Tsai, Hwei Ling Peng*

*Corresponding author for this work

Research output: Contribution to journalJournal Article peer-review

250 Scopus citations

Abstract

We have determined the entire DNA sequence of pLVPK, which is a 219-kb virulence plasmid harbored in a bacteremic isolate of Klebsiella pneumoniae. A total of 251 open reading frames (ORFs) were annotated, of which 37% have homologous genes of known function, 31% match the hypothetical genes in the GenBank database, and the remaining 32% are novel sequences. The obvious virulence-associated genes carried by the plasmid are the capsular polysaccharide synthesis regulator rmpA and its homolog rmpA2, and multiple iron-acquisition systems, including iucABCDiutA and iroBCDN siderophore gene clusters, Mesorhizobium loti fepBC ABC-type transporter, and Escherichia coli fecIRA, which encodes a Fur-dependent regulatory system for iron uptake. In addition, several gene clusters homologous with copper, silver, lead, and tellurite resistance genes of other bacteria were also identified. Identification of a replication origin consisting of a repA gene lying in between two sets of iterons suggests that the replication of pLVPK is iteron-controlled and the iterons are the binding sites for the repA to initiate replication and maintain copy number of the plasmid. Genes homologous with E. coli sopA/sopB and parA/parB with nearby direct DNA repeats were also identified indicating the presence of an F plasmid-like partitioning system. Finally, the presence of 13 insertion sequences located mostly at the boundaries of the aforementioned gene clusters suggests that pLVPK was derived from a sequential assembly of various horizontally acquired DNA fragments.

Original languageEnglish
Pages (from-to)189-198
Number of pages10
JournalGene
Volume337
Issue number1-2
DOIs
StatePublished - 04 08 2004
Externally publishedYes

Keywords

  • Heavy-metal resistance
  • IS
  • Iteron
  • LB
  • Luria-Bertani (medium)
  • ORF
  • Siderophore
  • Tellurite resistance
  • base pair(s)
  • bp
  • insertion sequence(s)
  • kb
  • kilobase(s)
  • open reading frame
  • ori
  • origin of DNA replication
  • rmpA

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