Serum microRNAs in clear cell carcinoma of the ovary

Angel Chao, Chyong Huey Lai, Hua Chien Chen, Chiao Yun Lin, Chia Lung Tsai, Yun Hsin Tang, Huei Jean Huang, Chen Tao Lin, Min Yu Chen, Kuang Gen Huang, Hung Hsueh Chou, Ting Chang Chang, Shu Jen Chen, Tzu Hao Wang*

*Corresponding author for this work

Research output: Contribution to journalJournal Article peer-review

28 Scopus citations

Abstract

Objective: To identify candidate microRNAs (miRNAs) in the serum of patients with clear cell carcinomas in monitoring disease progression. Materials and methods: The sera of patients with diagnosed ovarian clear cell carcinoma were collected from 2009 to 2012. Real-time quantitative polymerase chain reaction (PCR) analysis for 270 miRNAs was performed. To offset the potential extraction bias, an equal amount of Caenorhabditis elegans cel-miR-238 was added to each serum specimen before miRNA isolation. miRNA expression was analyzed using the δCt method, with cel-miR-238 as controls. Results: Twenty-one patients with clear cell carcinoma were included. In the discovery phase on four pairs of pre- and postoperative sera, 18 differentially expressed miRNAs were selected from 270 miRNAs. In the validation phase on an independent set of 11 pairs of pre- and postoperative sera, 4 miRNAs (hsa-miR-130a, hsa-miR-138, hsa-miR-187, and hsa-miR-202) were confirmed to be higher in the preoperative sera. In the application phase, hsa-miR-130a remained consistent with the different time points in seven of the 10 patients during clinical follow-up periods. More importantly, in three patients, hsa-miR-130a levels were elevated in early disease recurrences before CA125 was found to be elevated. Conclusion: Hsa-miR-130a may be a useful serum biomarker for detecting recurrence of ovarian clear cell cancer, and warrants further studies.

Original languageEnglish
Pages (from-to)536-541
Number of pages6
JournalTaiwanese Journal of Obstetrics and Gynecology
Volume53
Issue number4
DOIs
StatePublished - 01 12 2014

Bibliographical note

Publisher Copyright:
© 2014.

Keywords

  • Clear cell carcinoma
  • Hsa-miR-130a
  • MicroRNA
  • Ovarian cancer

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