Sex differences of oxidative stress to cholestatic liver and kidney injury in young rats

Kow Aung Chang, I. Chun Lin, Jiunn Ming Sheen, Yu Chieh Chen, Chih Cheng Chen, You Lin Tain, Chih Sung Hsieh, Li Tung Huang*

*Corresponding author for this work

Research output: Contribution to journalJournal Article peer-review

12 Scopus citations


Background: Sexual dimorphism plays a role in the liver and in renal injuries. However, whether sex is a risk factor in bile duct ligation (BDL) in young rats has never been examined. Methods: Six male and six female rats treated with BDL were sacrificed 2 weeks after surgery and were designated as BDL-M and BDL-F groups. The other six male and six female rats that received sham ligation were designated as sham-M and sham-F groups. Plasma biochemistry and liver and kidney asymmetric dimethylarginine (ADMA)-related molecules were examined. Results: Both BDL-M and BDL-F groups had elevated plasma aspartate transaminase (AST), alanine transaminase (ALT), bilirubin, and transforming growth factor-β1 levels. The BDL-F group had lower plasma AST and ALT levels than the BDL-M group. The BDL-M and BDL-F groups had elevated plasma ADMA levels. The cationic amino acid transporter 1 (CAT1) level was increased in the BDL-F group as compared to the sham-F group, whereas the CAT2 level was reduced in the both BDL-M and BDL-F groups. Conclusion: We found that young male rats were prone to higher degrees of biochemical liver and kidney injury to cholestasis. Sex differences in modulation of oxidative stress markers, such as ADMA, may play a role. Our results support careful monitoring and optimal treatment of cholestatic disease, especially in young male patients.

Original languageEnglish
Pages (from-to)95-101
Number of pages7
JournalPediatrics and Neonatology
Issue number2
StatePublished - 04 2013


  • asymmetric dimethylarginine
  • bile duct ligation
  • cationic amino acid transporter
  • nitric oxide
  • sexual dimorphism


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