Simultaneous detection of vitamin C and uric acid in human body fluids by capillary electrophoresis

M. L. Cheng, H. W.H. Lee, B. R.S. Hsu, T. Z. Liu, Daniel Tsun-Yee Chiu

Research output: Contribution to journalJournal Article peer-review


Vitamin C is a very important biological antioxidant and uric acid has also been proposed to have antioxidant funtion. An improved method for the simultaneous determination of ascorbic and uric acids by capillary etectrophoresis(CE) is developed. A 40 cm long fused-scilica capillary with an inside-diameter of 75 mm and 50 mM tricine running buffer, pH 8.8, was used. Both compounds were monitored by UV absorbance at 254 nm. A deproteinization step was required for samples with considerable protein content such as plasma. Addition of an equal volume of diethyl ether to the sample prior to the addition of 12% TCA gave complete deproteinization and allowed for excellent subsequent separation of vit. C and uric acid by CE. This method required less than 50μl of sample and had a detection limit of less than 2.0 μg/ml for both ascorbic acid and uric acid. Using this method, we found that diabetic patients had significantly (p<0.05) lower plasma vit. C level than normal controls. Whereas patients with acute anterior uveitis had significantly (p<0.05) lower vit. C level than normal controls but significantly higher uric acid level in their aqueous humor.

Original languageEnglish
Pages (from-to)A794
JournalFASEB Journal
Issue number5
StatePublished - 20 03 1998
Externally publishedYes


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