Abstract
Partition and production of the extracellular chitinase from Serratia marcescens were studied in PEG/dextran aqueous two-phase systems. The enzyme partitions into the bottom phase and the cells segregate into the top phase. The best system is 2% (w/v) PEG 20000 and 5% (w/v) dextran T500. The cell growth and enzyme production kinetics are similar in the aqueous two-phase system and in the polymer-free reference system. However, the maximum enzyme concentration in the former system is 1.5 times that in the latter one.
| Original language | English |
|---|---|
| Pages (from-to) | 783-788 |
| Number of pages | 6 |
| Journal | Biotechnology Techniques |
| Volume | 8 |
| Issue number | 11 |
| DOIs | |
| State | Published - 11 1994 |
| Externally published | Yes |