TY - GEN
T1 - Simultaneous reduction of specimeninduced spherical aberration and scattering effect in a Zeeman laser scanning confocal microscope
AU - Wu, Jheng Syong
AU - Chou, Chien
PY - 2011
Y1 - 2011
N2 - The scattering effect and spherical aberration induced by refractiveindex mismatch between a specimen and its surroundings degrade the resolution of sectioning images in a conventional confocal laser scanning microscope (CLSM). In our previous research, a Zeeman laser scanning confocal microscope (ZLSCM) was proposed. The proposed ZLSCM reduces specimen-induced scattering effect (H. F. Chang, et al., J. Microsc. 223, 26-32, 2006) and then specimen-induced spherical aberration (J. S. Wu, et al., Opt Express 18, 13136-13150, 2010). In this study, we demonstrate the ability of ZLSCM to simultaneously reduce specimen-induced spherical aberration and scattering effect. The method used is based on the features of the common path propagation of linear polarized photonpairs (LPPP) associated with the optical heterodyne detection. Three different kinds of gating (spatial coherence gating, polarization gating, and spatial pinhole gating) in ZLSCM not only reduce the wavefront deformation of the LPPP laser beam, but also increase the survivability of LPPP in scattering media. In experiments, we study the performance of ZLSCM on simultaneous reduction of scattering effect and spherical aberration as the LPPP laser beam is focused into a scattering medium at different depths. We compare the axial responses between ZLSCM and CLSM under the same conditions. These experiments verify that ZLSCM is able to simultaneously reduce specimen-induced spherical aberration and scattering effect.
AB - The scattering effect and spherical aberration induced by refractiveindex mismatch between a specimen and its surroundings degrade the resolution of sectioning images in a conventional confocal laser scanning microscope (CLSM). In our previous research, a Zeeman laser scanning confocal microscope (ZLSCM) was proposed. The proposed ZLSCM reduces specimen-induced scattering effect (H. F. Chang, et al., J. Microsc. 223, 26-32, 2006) and then specimen-induced spherical aberration (J. S. Wu, et al., Opt Express 18, 13136-13150, 2010). In this study, we demonstrate the ability of ZLSCM to simultaneously reduce specimen-induced spherical aberration and scattering effect. The method used is based on the features of the common path propagation of linear polarized photonpairs (LPPP) associated with the optical heterodyne detection. Three different kinds of gating (spatial coherence gating, polarization gating, and spatial pinhole gating) in ZLSCM not only reduce the wavefront deformation of the LPPP laser beam, but also increase the survivability of LPPP in scattering media. In experiments, we study the performance of ZLSCM on simultaneous reduction of scattering effect and spherical aberration as the LPPP laser beam is focused into a scattering medium at different depths. We compare the axial responses between ZLSCM and CLSM under the same conditions. These experiments verify that ZLSCM is able to simultaneously reduce specimen-induced spherical aberration and scattering effect.
KW - Heterodyne
KW - Microscope
KW - Photon-pair
KW - Scattering
KW - Spherical aberration
KW - Zeeman laser
UR - http://www.scopus.com/inward/record.url?scp=79952506429&partnerID=8YFLogxK
U2 - 10.3850/978-981-08-7615-9-FI06
DO - 10.3850/978-981-08-7615-9-FI06
M3 - 会议稿件
AN - SCOPUS:79952506429
SN - 9789810876159
T3 - ISOB 2011 - Proceedings of the 1st International Symposium ISOB 2011 - Proceedings of the 1st International Symposium on Bioengineering
SP - 111
EP - 119
BT - ISOB 2011 - Proceedings of the 1st International Symposium on Bioengineering
T2 - 1st International Symposium on Bioengineering, ISOB 2011
Y2 - 19 January 2011 through 19 January 2011
ER -