Single-Cell Impedance Flow Cytometry

Hongyan Liang, Huiwen Tan, Deyong Chen, Junbo Wang*, Jian Chen*, Min Hsien Wu*

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

2 Scopus citations

Abstract

This chapter reviews recent technical developments in the field of single-cell electrical property characterization. Firstly, three well-established characterization approaches including patch clamping, electrorotation, and dielectrophoresis are discussed and compared. Then, key developments of impedance flow cytometry, which are capable of high-throughput quantifying single-cell electrical properties, are presented. In addition, key cellular electrical properties quantified by these techniques are summarized and discussed. In the end, future research opportunities with technical innovations are indicated.

Original languageEnglish
Title of host publicationHandbook of Single-Cell Technologies
PublisherSpringer Singapore
Pages889-919
Number of pages31
ISBN (Electronic)9789811089534
ISBN (Print)9789811089527
DOIs
StatePublished - 01 01 2021

Bibliographical note

Publisher Copyright:
© Springer Nature Singapore Pte Ltd, part of Springer Nature 2022.

Keywords

  • Cellular bioelectrical characterization
  • Dielectrophoresis
  • Electrorotation
  • Impedance flow cytometry
  • Patch clamping
  • Single-cell analysis

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