Single-Cell Impedance Flow Cytometry

Hongyan Liang; Huiwen Tan; Deyong Chen; Junbo Wang; Jian Chen; Min Hsien Wu

doi:10.1007/978-981-10-8953-4_7

Single-Cell Impedance Flow Cytometry

Hongyan Liang
, Huiwen Tan
, Deyong Chen
, Junbo Wang^*
, Jian Chen^*
, Min Hsien Wu^*

^*Corresponding author for this work

Department of Biomedical Engineering

Research output: Chapter in Book/Report/Conference proceeding › Chapter › peer-review

3 Scopus citations

Abstract

This chapter reviews recent technical developments in the field of single-cell electrical property characterization. Firstly, three well-established characterization approaches including patch clamping, electrorotation, and dielectrophoresis are discussed and compared. Then, key developments of impedance flow cytometry, which are capable of high-throughput quantifying single-cell electrical properties, are presented. In addition, key cellular electrical properties quantified by these techniques are summarized and discussed. In the end, future research opportunities with technical innovations are indicated.

Original language	English
Title of host publication	Handbook of Single-Cell Technologies
Publisher	Springer Singapore
Pages	889-919
Number of pages	31
ISBN (Electronic)	9789811089534
ISBN (Print)	9789811089527
DOIs	https://doi.org/10.1007/978-981-10-8953-4_7
State	Published - 01 01 2021

Bibliographical note

Publisher Copyright:
© Springer Nature Singapore Pte Ltd, part of Springer Nature 2022.

Keywords

Cellular bioelectrical characterization
Dielectrophoresis
Electrorotation
Impedance flow cytometry
Patch clamping
Single-cell analysis

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10.1007/978-981-10-8953-4_7

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title = "Single-Cell Impedance Flow Cytometry",

abstract = "This chapter reviews recent technical developments in the field of single-cell electrical property characterization. Firstly, three well-established characterization approaches including patch clamping, electrorotation, and dielectrophoresis are discussed and compared. Then, key developments of impedance flow cytometry, which are capable of high-throughput quantifying single-cell electrical properties, are presented. In addition, key cellular electrical properties quantified by these techniques are summarized and discussed. In the end, future research opportunities with technical innovations are indicated.",

keywords = "Cellular bioelectrical characterization, Dielectrophoresis, Electrorotation, Impedance flow cytometry, Patch clamping, Single-cell analysis",

author = "Hongyan Liang and Huiwen Tan and Deyong Chen and Junbo Wang and Jian Chen and Wu, \{Min Hsien\}",

note = "Publisher Copyright: {\textcopyright} Springer Nature Singapore Pte Ltd, part of Springer Nature 2022.",

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AU - Liang, Hongyan

AU - Tan, Huiwen

AU - Chen, Deyong

AU - Wang, Junbo

AU - Chen, Jian

AU - Wu, Min Hsien

N1 - Publisher Copyright: © Springer Nature Singapore Pte Ltd, part of Springer Nature 2022.

PY - 2021/1/1

Y1 - 2021/1/1

N2 - This chapter reviews recent technical developments in the field of single-cell electrical property characterization. Firstly, three well-established characterization approaches including patch clamping, electrorotation, and dielectrophoresis are discussed and compared. Then, key developments of impedance flow cytometry, which are capable of high-throughput quantifying single-cell electrical properties, are presented. In addition, key cellular electrical properties quantified by these techniques are summarized and discussed. In the end, future research opportunities with technical innovations are indicated.

AB - This chapter reviews recent technical developments in the field of single-cell electrical property characterization. Firstly, three well-established characterization approaches including patch clamping, electrorotation, and dielectrophoresis are discussed and compared. Then, key developments of impedance flow cytometry, which are capable of high-throughput quantifying single-cell electrical properties, are presented. In addition, key cellular electrical properties quantified by these techniques are summarized and discussed. In the end, future research opportunities with technical innovations are indicated.

KW - Cellular bioelectrical characterization

KW - Dielectrophoresis

KW - Electrorotation

KW - Impedance flow cytometry

KW - Patch clamping

KW - Single-cell analysis

UR - https://www.scopus.com/pages/publications/85159487819

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SP - 889

EP - 919

BT - Handbook of Single-Cell Technologies

PB - Springer Singapore

ER -

Single-Cell Impedance Flow Cytometry

Abstract

Bibliographical note

Keywords

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