Single-layer graphene based SPR biochips for tuberculosis bacillus detection

Nan Fu Chiu*, Teng Yi Huang, Chun Chuan Kuo, Wei Che Lee, Min Hua Hsieh, Hsin Chih Lai

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

20 Scopus citations


This paper is intended to demonstrate a facile and effective method to construct single layer graphene films onto the self-assembled monolayer (SAM) at Au electrodes based surface plasmon resonance (SPR) biochips integrated loop-mediated isothermal amplification (LAMP) for tuberculosis bacillus (TB) detection. It is a novel Au-SAM-graphene nanocomposites and taking advantages of the striking properties of both graphene and Au film, fundamental understanding in hybrid material manipulation and new electrochemical properties can be obtained. The sensitivity of TB detection in the LAMP-based assay for the amplification of the Insertion Sequence 6110 (IS6110) samples was determined by a single-layer graphene/Au thin film and compared with that of a conventional Au/Cr-based SPR chips. The results show that a graphene/Au SPR offers a potentially powerful assay, with a highly sensitive analysis, that may be applicable as an important tool for bio-marker detection.

Original languageEnglish
Title of host publicationBiophotonics
Subtitle of host publicationPhotonic Solutions for Better Health Care III
StatePublished - 2012
EventBiophotonics: Photonic Solutions for Better Health Care III - Brussels, Belgium
Duration: 16 04 201219 04 2012

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
ISSN (Print)1605-7422


ConferenceBiophotonics: Photonic Solutions for Better Health Care III


  • Biosensor
  • Graphene
  • Insertion Sequence 6110 (IS6110)
  • Loop-mediated isothermal amplification (LAMP)
  • Self-assembled monolayer (SAM)
  • Surface plasmon resonance (SPR)
  • Tuberculosis bacillus (TB)


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