Soluble Dectin-1 as a tool to detect β-glucans

Lisa M. Graham, S. Vicky Tsoni, Janet A. Willment, David L. Williams, Philip R. Taylor, Siamon Gordon, Kevin Dennehy, Gordon D. Brown*

*Corresponding author for this work

Research output: Contribution to journalJournal Article peer-review

101 Scopus citations

Abstract

β-Glucans are structural components of fungal cell walls which are involved in the immune recognition of fungal pathogens and possess beneficial immunomodulatory activities in isolated form. Here we have developed a soluble chimeric form of the major mammalian β-glucan receptor, Dectin-1, and demonstrate its application for the detection and characterisation of soluble and insoluble β-glucans, including fungal particles, using ELISA, flow cytometric and fluorescence-based microscopy assays.

Original languageEnglish
Pages (from-to)164-169
Number of pages6
JournalJournal of Immunological Methods
Volume314
Issue number1-2
DOIs
StatePublished - 31 07 2006
Externally publishedYes

Keywords

  • Beta-glucan
  • C-type lectin
  • Dectin-1
  • ELISA
  • Innate immunity

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