Soluble Dectin-1 as a tool to detect β-glucans

Lisa M. Graham; S. Vicky Tsoni; Janet A. Willment; David L. Williams; Philip R. Taylor; Siamon Gordon; Kevin Dennehy; Gordon D. Brown

doi:10.1016/j.jim.2006.05.013

Soluble Dectin-1 as a tool to detect β-glucans

Lisa M. Graham, S. Vicky Tsoni, Janet A. Willment, David L. Williams, Philip R. Taylor, Siamon Gordon, Kevin Dennehy, Gordon D. Brown^*

^*Corresponding author for this work

Research output: Contribution to journal › Journal Article › peer-review

107 Scopus citations

Abstract

β-Glucans are structural components of fungal cell walls which are involved in the immune recognition of fungal pathogens and possess beneficial immunomodulatory activities in isolated form. Here we have developed a soluble chimeric form of the major mammalian β-glucan receptor, Dectin-1, and demonstrate its application for the detection and characterisation of soluble and insoluble β-glucans, including fungal particles, using ELISA, flow cytometric and fluorescence-based microscopy assays.

Original language	English
Pages (from-to)	164-169
Number of pages	6
Journal	Journal of Immunological Methods
Volume	314
Issue number	1-2
DOIs	https://doi.org/10.1016/j.jim.2006.05.013
State	Published - 31 07 2006
Externally published	Yes

Keywords

Beta-glucan
C-type lectin
Dectin-1
ELISA
Innate immunity

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10.1016/j.jim.2006.05.013

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title = "Soluble Dectin-1 as a tool to detect β-glucans",

abstract = "β-Glucans are structural components of fungal cell walls which are involved in the immune recognition of fungal pathogens and possess beneficial immunomodulatory activities in isolated form. Here we have developed a soluble chimeric form of the major mammalian β-glucan receptor, Dectin-1, and demonstrate its application for the detection and characterisation of soluble and insoluble β-glucans, including fungal particles, using ELISA, flow cytometric and fluorescence-based microscopy assays.",

keywords = "Beta-glucan, C-type lectin, Dectin-1, ELISA, Innate immunity",

author = "Graham, \{Lisa M.\} and Tsoni, \{S. Vicky\} and Willment, \{Janet A.\} and Williams, \{David L.\} and Taylor, \{Philip R.\} and Siamon Gordon and Kevin Dennehy and Brown, \{Gordon D.\}",

year = "2006",

month = jul,

day = "31",

doi = "10.1016/j.jim.2006.05.013",

language = "英语",

volume = "314",

pages = "164--169",

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T1 - Soluble Dectin-1 as a tool to detect β-glucans

AU - Graham, Lisa M.

AU - Tsoni, S. Vicky

AU - Willment, Janet A.

AU - Williams, David L.

AU - Taylor, Philip R.

AU - Gordon, Siamon

AU - Dennehy, Kevin

AU - Brown, Gordon D.

PY - 2006/7/31

Y1 - 2006/7/31

N2 - β-Glucans are structural components of fungal cell walls which are involved in the immune recognition of fungal pathogens and possess beneficial immunomodulatory activities in isolated form. Here we have developed a soluble chimeric form of the major mammalian β-glucan receptor, Dectin-1, and demonstrate its application for the detection and characterisation of soluble and insoluble β-glucans, including fungal particles, using ELISA, flow cytometric and fluorescence-based microscopy assays.

AB - β-Glucans are structural components of fungal cell walls which are involved in the immune recognition of fungal pathogens and possess beneficial immunomodulatory activities in isolated form. Here we have developed a soluble chimeric form of the major mammalian β-glucan receptor, Dectin-1, and demonstrate its application for the detection and characterisation of soluble and insoluble β-glucans, including fungal particles, using ELISA, flow cytometric and fluorescence-based microscopy assays.

KW - Beta-glucan

KW - C-type lectin

KW - Dectin-1

KW - ELISA

KW - Innate immunity

UR - https://www.scopus.com/pages/publications/33747047848

U2 - 10.1016/j.jim.2006.05.013

DO - 10.1016/j.jim.2006.05.013

M3 - 文章

C2 - 16844139

AN - SCOPUS:33747047848

SN - 0022-1759

VL - 314

SP - 164

EP - 169

JO - Journal of Immunological Methods

JF - Journal of Immunological Methods

IS - 1-2

ER -

Soluble Dectin-1 as a tool to detect β-glucans

Abstract

Keywords

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