Somatostatin-14 and somatostatin-28 induce opposite effects on potassium currents in rat neocortical neurons

H. L. Wang, C. Bogen, T. Reisine*, M. Dichter

*Corresponding author for this work

Research output: Contribution to journalJournal Article peer-review

154 Scopus citations


The prosomatostatin-derived peptides somatostatin-14 (Som-14) and somatostatin-28 (Som-28) are believed to act as neurotransmitters in the central nervous system. To examine possible mechanisms by which these peptides induce their physiological actions in brain, the effects of Som-14 and Som-28 on voltage-dependent K+ currents in rat cerebral cortical neurons in culture were examined by using whole-cell patch-clamp techniques. Som-14 increased a delayed rectifier K+ current (I(K)) in the cortical neurons, while Som-28 reduced I(K) in the neurons, both in a concentration-dependent manner. Som-14 and Som-28 could induce opposite changes in I(K) in the same neurons. Elevating intracellular cAMP in the cortical neurons did not modify the effects of Som-14 or Som-28 on I(K), indicating that the peptides can regulate this ionic current through cAMP-independent mechanisms. Pretreatment of the neocortical cells with pertussis toxin, which inactivates inhibitory GTP-binding proteins, abolished both Som-14 and Som-28 modulation of I(K), indicating that Som-14 and Som-28 receptors are coupled to I(K) via GTP-binding proteins. These studies show that Som-14 and Som-28 can induce opposite biological effects, suggesting that Som-14 and Som-28, acting through distinct receptors, may function as different neurotransmitters or neuromodulators.

Original languageEnglish
Pages (from-to)9616-9620
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Issue number23
StatePublished - 1989
Externally publishedYes


  • adenylate cyclase
  • cerebral cortex
  • potassium channels
  • prosomatostatin


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