TY - JOUR
T1 - Somatostatin and macrophage function
T2 - modulation of hydrogen peroxide, nitric oxide and tumor necrosis factor release
AU - Chao, Tzu Chieh
AU - Cheng, Ho Pin
AU - Walter, Robert J.
PY - 1995/7/21
Y1 - 1995/7/21
N2 - Recent studies have shown that somatostatin modulates lymphocyte function, but the effects of somatostatin on macrophage function are not clearly defined. In the present study, peritoneal macrophages (M∅) obtained from male rats were treated in vitro with somatostatin or octreotide and their effects on the release of hydrogen peroxide (H2O2), nitrite, and tumor necrosis factor (TNF) determined. Macrophages treated with somatostatin (10-9 M to 10-7 M) or octreotide (10-8 M and 10-7 M) released significantly greater amounts of PMA-stimulated H2O2 than did the untreated controls. In addition, 10-9 M of somatostatin significantly enhanced PMA-stimulated H2O2 release by LPS-treated M∅. Octreotide had no effect on H2O2 release by LPS-treated M∅. At concentrations of 10-14 M, 10-13 M, or greater than 10-8 M, somatostatin or octrectide suppressed nitrite release by M∅. Somatostatin or or octreotide did not affect nitrite release by LPS-treated M∅. On the other hand, M∅ treated with 10-11 M of somatostatin or octreotide released greater amounts of TNF than did the untreated controls. In contrast, TNF release by M∅ treated with 10-9 M to 10-5 M of somatostatin or 10-7 M to 10-5 M of octreotide was less than that of the controls. Anti-TNF antibody (1:1000) caused a reduction in the release of H2O2 and nitrite. These findings demonstrate that somatostatin and octreotide modulate the release of H2O2, nitric oxide, and TNF by M∅ depending on the concentration of hormones used.
AB - Recent studies have shown that somatostatin modulates lymphocyte function, but the effects of somatostatin on macrophage function are not clearly defined. In the present study, peritoneal macrophages (M∅) obtained from male rats were treated in vitro with somatostatin or octreotide and their effects on the release of hydrogen peroxide (H2O2), nitrite, and tumor necrosis factor (TNF) determined. Macrophages treated with somatostatin (10-9 M to 10-7 M) or octreotide (10-8 M and 10-7 M) released significantly greater amounts of PMA-stimulated H2O2 than did the untreated controls. In addition, 10-9 M of somatostatin significantly enhanced PMA-stimulated H2O2 release by LPS-treated M∅. Octreotide had no effect on H2O2 release by LPS-treated M∅. At concentrations of 10-14 M, 10-13 M, or greater than 10-8 M, somatostatin or octrectide suppressed nitrite release by M∅. Somatostatin or or octreotide did not affect nitrite release by LPS-treated M∅. On the other hand, M∅ treated with 10-11 M of somatostatin or octreotide released greater amounts of TNF than did the untreated controls. In contrast, TNF release by M∅ treated with 10-9 M to 10-5 M of somatostatin or 10-7 M to 10-5 M of octreotide was less than that of the controls. Anti-TNF antibody (1:1000) caused a reduction in the release of H2O2 and nitrite. These findings demonstrate that somatostatin and octreotide modulate the release of H2O2, nitric oxide, and TNF by M∅ depending on the concentration of hormones used.
KW - Lipopolysaccharide
KW - Octreotide
KW - Rat
UR - http://www.scopus.com/inward/record.url?scp=0029649768&partnerID=8YFLogxK
U2 - 10.1016/0167-0115(95)00051-C
DO - 10.1016/0167-0115(95)00051-C
M3 - 文章
C2 - 8570854
AN - SCOPUS:0029649768
SN - 0167-0115
VL - 58
SP - 1
EP - 10
JO - Regulatory Peptides
JF - Regulatory Peptides
IS - 1-2
ER -